DIA based Protein Quantitative Service at MtoZ Biolabs

MtoZ Biolabs offers DIA based protein quantitative service, utilizing the advanced AB SCIEX Triple-TOF 5600+ high-resolution mass spectrometry system. This system combines high-speed scanning, enhanced quantitative sensitivity, and the advantages of quadrupole tandem mass spectrometry, integrating high resolution, precise mass stability, ultra-high sensitivity, and rapid scanning capabilities. It significantly improves the accuracy and stability of quantitative analysis, ensuring excellent performance even in complex biological samples.

 

Data-Independent acquisition (DIA) is a data acquisition mode that does not rely on precursor ion intensity, based on electrostatic field orbitrap mass spectrometry. In this method, all ionized compounds within a specified mass range of a given sample are fragmented and analyzed for quantification following mass spectrometry analysis. The process precisely collects precursor ion fragments and rapidly scans the mass spectrometry data, leading to enhanced quantitative accuracy and better reproducibility. A key application of DIA-based quantitative proteome analysis is the SWATH (Sequential Window Acquisition of All Theoretical Fragment Ion Spectra) technique, which integrates high-throughput shotgun proteomics with precise parallel reaction monitoring (PRM) quantification, offering unparalleled depth and reliability in protein quantification.

 

Analysis Workflow of MtoZ Biolabs' DIA based Protein Quantitative Service

The DIA based protein quantitative service provided by MtoZ Biolabs utilizes advanced mass spectrometry systems and optimized experimental workflows. The following outlines the complete workflow of this service, spanning all stages, from sample preparation through to final data analysis, with each stage adhering to rigorous scientific standards.

 

1. Sample Preparation

Based on the client's specific requirements, MtoZ Biolabs offers tailored sample preparation protocols for different sample types, including cells, tissues, or bodily fluids. Samples typically undergo processes such as lysis, protein extraction, contaminant removal, and protein concentration measurement. To preserve protein integrity and stability, precautions are taken to prevent degradation, including freezing the samples when necessary.

 

2. Protein Extraction and Enzymatic Digestion

MtoZ Biolabs uses highly effective protein extraction techniques to maximize the yield of target proteins from complex biological samples. Following protein extraction, optimized enzymatic digestion (typically using trypsin) is performed to break down proteins into peptide fragments. The efficiency of the enzymatic digestion process is crucial for the success of subsequent mass spectrometry analysis. By precisely controlling the digestion conditions, MtoZ Biolabs ensures high-quality peptide fragments are produced.

 

3. Liquid Chromatography Separation

Liquid chromatography (LC) is a critical step in DIA based protein quantitative service. We employ high-resolution LC systems, such as NanoLC, to separate peptide fragments. This separation reduces the complexity of the mass spectrometry data, allowing for more precise analysis. The core of this step is achieving accurate separation, ensuring that each peptide is sufficiently analyzed.

 

4. Mass Spectrometry Analysis

The technology of DIA based protein quantitative service differs from traditional data-dependent acquisition (DDA) by acquiring ion signals from all ions simultaneously, rather than selectively measuring a subset of ions. This increases the comprehensiveness and reliability of the data. MtoZ Biolabs uses advanced mass spectrometers, such as Q-TOF and Orbitrap, for peptide detection and identification. In DIA mode, the mass spectrometer collects data over a wide mass range, allowing for the capture of a broader spectrum of peptide and protein information.

 

5. Data Processing and Quantitative Analysis

After mass spectrometry analysis, advanced data analysis software is used to process the DIA data. This typically includes peptide identification and quantification based on specific databases. DIA technology allows for precise quantification of protein abundance by identifying variations in peptide intensities. MtoZ Biolabs employs software such as MaxQuant and Spectronaut, which effectively denoise, normalize, and quantify complex datasets.

 



 

Applications

MtoZ Biolabs' DIA based protein quantitative service is extensively utilized across various research domains, demonstrating substantial potential particularly in biomarker discovery, tumor subtyping, and agricultural and forestry applications.

 

1. Biomarker Research

In the field of biomedical research, the discovery and validation of biomarkers are crucial for clinical diagnosis, disease prediction, and personalized treatment. MtoZ Biolabs' DIA based protein quantitative service provides robust technical support for biomarker research. By utilizing DIA technology, we can perform high-throughput and high-precision protein quantification in complex clinical samples, significantly enhancing the reliability and sensitivity of biomarker screening. The high coverage and excellent quantitative accuracy of DIA make it an especially effective tool for large-scale clinical cohort studies, enabling simultaneous qualitative and quantitative analysis of hundreds of proteins.

 

2. Tumor Tissue Disease Subtyping

MtoZ Biolabs offers an advanced solution for tumor research by combining DIA technology with phosphoproteomics (DIA+phosphoproteomics). This approach allows for precise quantification of proteins in tumor tissues while minimizing data loss due to technical variability during tumor subtype classification, ensuring both efficiency and reliability in the analysis. By reducing data gaps, DIA technology increases the proportion of valid data, facilitating more accurate tumor subtype classification and supporting flexible experimental designs that are not constrained by assumptions regarding sample grouping.

 

3. Comparative Studies in Agriculture and Forestry

MtoZ Biolabs' DIA based protein quantitative service enables researchers to conduct efficient and systematic protein expression profiling in comparative studies of multiple plant or animal species. By leveraging DIA technology, researchers can comprehensively analyze protein data from complex biological samples, establish extensive expression profile databases, and drive advancements in gene function research, stress resistance studies, and species improvement initiatives.

 

Why Choose MtoZ Biolabs?

Our primary advantages include:

1. Advance Analysis Platform

MtoZ Biolabs' advanced DIA-based quantitative proteome analysis platform guarantees reliable, fast, and highly accurate analysis service.

 

2. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

3. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all DIA-based quantitative proteome analysis data, providing clients with a comprehensive data report.

 

What Could Be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

5. Bioinformatics Analysis

6. Raw Data Files