Detection of Host Cell Proteins (HCP) Based on PRM
Host Cell Proteins (HCP) are impurities in the form of proteins that originate from production cells during the manufacturing process of biopharmaceuticals. These impurities can potentially compromise the safety, purity, and efficacy of the final product, highlighting the critical need for their detection and quantification. In this context, we utilize Parallel Reaction Monitoring (PRM) technology for HCP detection.
PRM Technology
PRM is a targeted mass spectrometry technique employed for the quantitative analysis of specific proteins or peptides. This approach leverages the high resolution and sensitivity of mass spectrometers to identify and quantify distinct mass spectrometry signals from pre-selected peptides or proteins.
Steps for HCP Detection via PRM
1. Sample Preparation
Initially, proteins are extracted from biopharmaceutical samples and subsequently digested into peptides enzymatically.
2. PRM Experiment
During the PRM experiment, a set of specific peptides related to the target HCP is pre-determined and analyzed using a mass spectrometer. This targeted approach enhances both the sensitivity and specificity of the detection process.
3. Data Analysis
Following data acquisition from the mass spectrometer, rigorous data processing and analysis are conducted to confirm and quantify the presence of HCP.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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