Deoxycorticosterone Analysis Service
- Identifying new disease biomarkers
- Improving disease diagnosis
- Accelerating the discovery of novel therapeutics
- Revealing fresh insights into disease mechanisms
- Animal tissue ≥ 100 mg
- Plant tissue ≥ 200 mg
- Serum/ Plasma ≥ 200 μL
- Urine ≥ 2 mL
Deoxycorticosterone (DOC) is a hormone secreted by the adrenal cortex, typically synthesized from progesterone through the catalytic action of the enzyme 21-hydroxylase (CYP21A2). Its chemical structure is C₂₁H₃₀O₃, characterized by a typical steroid backbone consisting of four carbon rings (A, B, C, D rings). The structure of DOC includes an aldehyde group (-CH₂OH) at the 21st carbon position and a ketone group (-C=O) at the 3rd position. Like other steroid hormones, it has a double bond between the 4th and 5th carbons in the A ring. DOC is an important intermediate in the aldosterone synthesis pathway and plays a role in regulating water and electrolyte balance in the body. The levels of DOC are regulated by the renin-angiotensin system, with its synthesis increasing especially in conditions of fluid deficiency or low blood pressure. The metabolism of deoxycorticosterone primarily involves the enzyme 11β-hydroxylase (CYP11B1), which catalyzes the production of more active compounds such as 11-deoxycortisol and aldosterone. Aldosterone, a key member of the mineralocorticoid hormone family, has stronger effects on sodium reabsorption and water metabolism regulation. In animal models and human samples, the concentration of DOC can serve as a marker for studying mineral metabolism, adrenal cortex function, and blood pressure regulation. Changes in DOC levels provide crucial insights into the mechanisms underlying diseases, particularly in the research of hypertension and electrolyte imbalances. Moreover, analyzing DOC metabolites, such as aldosterone, can help in diagnosing adrenal gland disorders, such as primary aldosteronism, and other metabolic disturbances. Using mass spectrometry and other high-sensitivity analytical methods to quantify DOC and its metabolites not only enhances our understanding of human mineral metabolism but also offers potential new research directions in fields such as agriculture.
Figure 1. The Structure of Deoxycorticosterone
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider, providing advanced metabolomics, proteomics and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. MtoZ Biolabs utilizes an LC-MS-based metabolomics research platform for the analysis of deoxycorticosterone. We ensure the accuracy of the quantification of deoxycorticosterone and its metabolites, as well as the comprehensiveness and precision of metabolite identification. If you are interested in our deoxycorticosterone analysis service, please feel free to contact us at any time, and we will be fully committed to providing you with high-quality analytical services and technical support.
Analysis Workflow
Service Advantages
1. Advance Analysis Platform: MtoZ Biolabs established an advanced deoxycorticosterone analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Extensive data coverage with rigorous quality control. Our advanced analysis platform consolidates all deoxycorticosterone analysis data, delivering clients a detailed and comprehensive data report.
4. Quick Turnaround: We offer a fast analysis process to ensure that clients receive high-quality data within a short time.
Applications
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, tissues, and other biological samples. It is requested to select more than 3 materials with the same condition for each sample.
2. Sample Volume
3. Sample Preservation
Store at -80°C to maintain stability.
Note: Provide details on sample collection and handling.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on Deoxycorticosterone
4. Raw Data
5. Custom Analysis Report
With our deoxycorticosterone analysis service, we aim to streamline your research and product development processes by providing precise and trustworthy results.
How to order?
