Antibody Amino Acid Sequencing Service

The core of antibody full amino acid sequencing involves determining the amino acid sequence of antibody proteins using mass spectrometry (MS) technology. MS technology employs electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI) to ionize proteins and measure their mass-to-charge ratio (m/z). The process begins with enzymatic digestion of the protein into smaller peptide fragments. These peptides are then analyzed using MS to obtain their mass data and fragmentation information. Finally, bioinformatics tools are used to assemble these data, reconstructing the complete amino acid sequence of the antibody.

 

MtoZ Biolabs has developed an efficient, fast, and accurate antibody full amino acid sequencing platform based on high-throughput mass spectrometry (MS) technology. Our team of experienced scientists and technical experts has deep expertise in antibody engineering and optimization research. We are committed to providing high-quality, efficient services to advance antibody full amino acid sequencing research and antibody-related drug development. Free project evaluation, welcome to learn more details! Our technical specialists are available to provide a free business assessment.

 

Analysis Workflow

1. Sample Preparation

Purification: Purifying the antibody sample to ensure it is free from contaminants and of high quality.

Quantification: Ensuring the sample amount is typically above 100 micrograms.

 

2. Enzymatic Digestion

Enzyme Selection: Using specific proteases, such as trypsin, to cleave the antibody into smaller peptides.

Digestion Conditions: Controlling the digestion conditions to ensure the peptide lengths are suitable for subsequent mass spectrometry analysis.

 

3. Mass Spectrometry Analysis

Ionization: Ionizing the peptides using high-resolution mass spectrometry (HRMS).

Mass Analysis: Utilizing tandem mass spectrometry (MS/MS) to generate mass data of the peptides.

 

4. Data Interpretation

Sequence Assembly: Using specialized software tools to interpret the mass spectrometry data and reconstruct the amino acid sequence of the antibody.

Sequence Verification: Ensuring the sequence information of all peptides is accurate.

 

5. Validation and Reporting

Validation: Performing further experiments to validate the sequencing results and ensure data reliability.

Reporting: Generating a detailed sequencing report, including the complete amino acid sequence information.

 

Service Advantages

1. Comprehensive Sequence Information

We provide the entire amino acid sequence of both the light and heavy chains of the antibody, including variable (V) and constant (C) regions. Enables a thorough understanding of the antibody's structure, which is crucial for studying its functional properties and interactions.

 

2. Rigorous Quality Control and Validation

Our service ensures consistency across different production batches by providing a detailed sequence comparison and meets stringent regulatory requirements for antibody drugs by providing comprehensive sequence data.

 

3. High-resolution Analytical Capabilities

High-resolution mass spectrometry (HRMS) and tandem mass spectrometry (MS/MS) are utilized to achieve high sensitivity and accuracy in sequence determination and sophisticated bioinformatics tools are employed to accurately reconstruct the full amino acid sequence from mass spectrometry data.

 

Sample Results

Purification, structural analysis and stability of antioxidant peptides from purple wheat bran

 



Zhao Y, et al. BMC Chem. 2020.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Antibody Full Amino Acid Sequencing

4. Mass Spectrometry Images

5. Raw Data