Animal Cell-derived Exosome Isolation and Development Service

Exosomes are nanoscale extracellular vesicles (30–150 nm) released from intracellular multivesicular bodies (MVBs) and are extensively distributed across various animal cell types. Animal cell-derived exosomes play pivotal roles in intercellular communication, making them invaluable in elucidating disease mechanisms, biomarker discovery, and developing drug delivery systems.

 



Shi, S. et al. Int. J. Mol. Sci. 2023.

Figure 1. Biogenesis of Exosomes and Distinction from Other Extracellular Vesicles

 

However, challenges such as complex cell culture media backgrounds, overlapping exosome size distributions, and low abundance complicate their isolation and analysis. To overcome these obstacles, MtoZ Biolabs offers a specialized Animal Cell-derived Exosome Isolation and Development Service that covers comprehensive and reliable solutions from initial sample processing to final functional validation.

 

Services at MtoZ Biolabs

MtoZ Biolabs' Animal Cell-derived Exosome Isolation and Development Service encompasses the entire workflow, from exosome extraction to extensive functional assessments, tailored to meet various research and application needs. Key services include:

1. Standardized Isolation of Animal Cell-derived Exosomes

Employing advanced methodologies such as differential ultracentrifugation, density gradient ultracentrifugation, and immunoaffinity capture, we achieve consistent and reproducible isolation of high-quality exosomes from diverse sources of mammalian cells.

 

2. High-Purity Exosome Purification

Leveraging sophisticated technologies including Size Exclusion Chromatography (SEC) and nanoporous membranes, we efficiently eliminate cellular debris and contaminating proteins, significantly enhancing the purity and integrity of exosomal preparations.

 

3. Comprehensive Exosome Characterization

Our Animal Cell-derived Exosome Isolation and Development Service integrates nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), dynamic light scattering (DLS), and Western blotting (CD9, CD63, CD81) for detailed and precise characterization of isolated exosomes.

 

4. Multi-omics Analysis of Exosomes

Utilizing state-of-the-art technologies, we perform comprehensive proteomic, lipidomic, and nucleic acid (miRNA, lncRNA) analyses, uncovering the molecular functions and biological networks associated with exosomes.

 

5. Functional Validation and Exosome Engineering

We offer tailored functional studies including cellular uptake assays, delivery efficiency tests, and assessment of biological activities, as well as advanced engineering methods for customized applications, such as drug delivery and therapeutic interventions.

 

Service Advantages

MtoZ Biolabs’ Animal Cell-derived Exosome Isolation and Development Service offers distinct technical advantages:

• Integration of multiple isolation platforms ensuring superior purity and integrity.
• Standardized operating procedures guaranteeing consistency across experiments.
• Highly sensitive analytical tools providing comprehensive quality and functionality evaluation.
• Robust exosome engineering capabilities suitable for diverse research and clinical application scenarios.
• Dedicated professional team offering customized, one-on-one project solutions.

 

Applications

Our Animal Cell-derived Exosome Isolation and Development Service delivers high-quality exosomes addressing core challenges such as inconsistent exosome sources, low purity, and undefined functions, supporting various applications including:

1. Disease Biomarker Discovery and Liquid Biopsy

Exosomes are rich in disease-specific miRNAs and proteins, ideal for biomarker screening in cancers, cardiovascular diseases, and immune disorders. Our high-purity isolation technology ensures accurate diagnostic biomarker discovery.

 

2. Drug Delivery Systems Development

Exosomes serve as natural nanocarriers for therapeutic agents including RNAs, proteins, and small molecules. We offer cargo loading optimization and surface modifications, enabling efficient, targeted drug delivery applications.

 

3. Immunomodulation and Therapeutics

Exosomes possess potent immunomodulatory properties useful in treating autoimmune diseases, inflammation, and cancers. Our services include the engineering of exosomes for enhanced immunoregulatory functions.

 

4. Regenerative Medicine and Tissue Repair

Exosomes containing bioactive molecules support regeneration in damaged tissues like cardiac, neural, and hepatic tissues. MtoZ Biolabs delivers functional exosomes optimized for tissue engineering applications.

 

5. Cell Therapy Enhancement

Engineered exosomes can improve the therapeutic efficacy of stem cell and immune cell therapies. We customize exosomes to boost cell transplantation survival rates and therapeutic outcomes, promoting industrialization of cell-based treatments.

 

 

Su, S. J. et al. Stem Cell Rep. 2021.

Figure 1. Multifunctional Mechanisms and Cardiac Repair Applications of Animal Cell-derived Exosomes

 

FAQ

Q1. What types of animal cell-derived exosomes does MtoZ Biolabs isolate?

We isolate exosomes from diverse cell types including stem cells (MSCs, iPSCs), immune cells (T/B cells, macrophages), tumor cells, neurons, fibroblasts, and can also offer customized isolation solutions for specific cell types.

 

Q2. Can exosomes be loaded with drugs or nucleic acids?

Yes, we provide loading services for cargos such as siRNA, miRNA, proteins, and antibodies, accompanied by validation of delivery efficiency and functional activity.

 

For reliable and functionally validated animal cell-derived exosomes, choose MtoZ Biolabs' Animal Cell-derived Exosome Isolation and Development Service to accelerate your research and clinical projects effectively.