Acetyl CoA Analysis Service

    MtoZ Biolabs: Analysis of Acetyl-CoA and Related Substances

    Acetyl-CoA, an activated form of acetate, is formed by an acetyl group (CH3CO-)  bonding to the thiol group of coenzyme A via a high-energy thioester bond. It is product of the decarboxylation of pyruvate produced by β-oxidation and glycolysis of fatty acid (FA). In the initial step of the tricarboxylic acid cycle (TAC), the acetyl group is transferred to oxaloacetate to form citrate, and this cycle is also called the "citric acid cycle". MtoZ Biolabs utilizes ACQUITY UPLC/TripleQuad5500 (Waters/AB Sciex) for the quantitative and qualitative analysis of acetyl-CoA and its related substances.

     

    Detection of Acetyl-CoA and Related Substances at MtoZ Biolabs

    Our technicians, with extensive technical expertise, use ACQUITY UPLC/TripleQuad5500 (Waters/AB Sciex) alongside acetyl-CoA and related substance standards and isotopic standards for precise quantitative and qualitative analysis:

     

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    Figure 1. Services for Analysis of Acetyl-CoA and Related Substances

     

    Sample Submission Requirements

    Serum, plasma, urine, bile, bile acids; cell, animal tissues including liver, brain, and feces; plants, yeast, microorganisms, etc.

    1. Sample requirements

    (1) Blood, bile, etc.: 10 µL

    (2) Various tissues: 10 mg

    (3) Feces, etc.: 10 mg

    For other sample types and sizes, please contact MtoZ Biolabs salesman.

     

    2. Sample Transportation

    Considering the evaporation rate of 3-4 kg of dry ice per day, ensure ample dry ice used for mailing (preferably larger dry ice pieces as they evaporate slower), and sealed in foam boxes. Opt for the fastest possible mailing method to reduce sample degradation during transport. 

     

    Deliverables

    1. Experimental Procedures

    2. Relevant Mass Spectrometry Parameters

    3. Quantitative or Qualitative Information of Identified Substances

    4. Mass Spectrometry Images

    5. Raw Data

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