4D-Ubiquitination Proteomics Service

Protein ubiquitination is a critical post-translational modification, where one or more ubiquitin molecules are covalently attached to lysine residues of target proteins via an enzymatic cascade involving E1, E2, and E3 enzymes. This modification often leads to protein degradation through the ubiquitin-proteasome system (UPS), regulating protein stability and expression. Ubiquitination also plays key roles in various cellular processes, including cell proliferation, apoptosis, endocytosis, and immune regulation.

 



Xu, L. D. et al. PLoS ONE. 2012.

Figure 1. Roles of Protein Ubiquitination and Degradation Kinetics in Biological Oscillations

 

Ubiquitination-modified lysine residues are covalently linked to two glycine (K-ε-GG) residues of ubiquitin, resulting in a mass shift of 114.1 Da. Mass spectrometry can detect this mass shift with high precision to identify ubiquitinated peptides and modification sites. MtoZ Biolabs offers 4D Ubiquitination Proteomics Service, based on the timsTOF Pro mass spectrometry platform, utilizing 4D Label-Free proteomics technology. This method, combined with ion mobility separation, can effectively distinguish isomeric ubiquitinated peptides based on their molecular shape and size, greatly enhancing identification depth, sensitivity, and throughput. It is particularly suited for the analysis of low-abundance samples in differential proteomics studies. 

 

Analysis Workflow

 

 

Service Advantages

 



 

Sample Submission Requirements

 



For more sample details, please consult our technical team.

 

Applications

1. Neurodegenerative Disease Research: Investigate the role of ubiquitination in the regulation of neurodegenerative diseases.

 

2. Cancer Pathogenesis Research: Analyze ubiquitination patterns in tumor cells to explore their roles in cancer development and progression.

 

3. Pharmacological and Drug Target Research: Monitor ubiquitination changes in response to drug treatments to validate drug targets and mechanisms.

 

4. Immune Regulation Studies: Explore ubiquitination's involvement in immune system regulation, revealing its role in immune responses.

 

Our 4D-Ubiquitination Proteomics Service provides high-quality data support, advancing your scientific research and applications. For any inquiries or requirements, please contact us.