4D-Acetylation Proteomics Service

Protein acetylation is one of the most prevalent types of acylation modifications in cells, primarily occurring at the ε-NH₂ group of lysine residues. This modification is catalyzed by acetyltransferases, which covalently attach acetyl groups (such as acetyl-CoA) to lysine residues, resulting in a molecular weight increase of 42.01 Da. Protein acetylation plays a critical role in regulating metabolic pathways and enzyme activity, and it is dynamically controlled by both acetyltransferases and deacetylases in response to changing metabolic conditions. Acetylation is commonly found on metabolic enzymes and is involved in the regulation of metabolic pathways, influencing downstream metabolite alterations. Thus, accurately identifying acetylation sites is crucial for understanding the metabolic dynamics within cells.

 



Qiu, W. R. et al. Front. Bioeng. Biotechnol. 2019.

Figure 1. An illustration to Show the Acetylation Protein

 

MtoZ Biolabs offers 4D Acetylation Proteomics Service, utilizing the timsTOF Pro mass spectrometry platform integrated with ion mobility separation technology. This method distinguishes acetylated isomeric peptides based on their molecular shape and cross-sectional area, significantly enhancing both the depth and precision of acetylation modification identification. It is particularly suited for the analysis of small sample volumes, allowing researchers to investigate the roles of acetylation in cellular metabolism, gene expression, and signal transduction.

 

Technical Principles

4D acetylation proteomics leverages next-generation mass spectrometry combined with ion mobility separation to achieve high-precision identification of acetylated peptides. Compared to traditional approaches, the 4D method incorporates a time dimension, greatly improving both coverage depth and sensitivity. This advanced technique accurately detects acetylation sites, providing researchers with a comprehensive view of acetylation's role in diverse biological processes.

 

Analysis Workflow

 



 

Service Advantages

 



 

Sample Submission Requirements

 



For more sample details, please consult our technical team.

 

Applications

• Regulation of energy metabolism
• Metabolic syndrome research
• Fungal-bacterial studies

 

MtoZ Biolabs' 4D Acetylation Proteomics Service offers an efficient and precise platform for the in-depth analysis of protein acetylation modifications. This service empowers researchers to explore the function of acetylation across a variety of biological processes. Our cutting-edge technology and expert services will help you achieve breakthroughs in your research. Please contact us for more details and tailored solutions.