3-Beta-Hydroxy-5-Cholestenoate Analysis Service

    3-beta-hydroxy-5-cholestenoate, with the chemical formula C31H54O3Si, is a metabolic intermediate in the biosynthesis of bile acids from cholesterol. Its structure is characterized by the presence of a hydroxyl (-OH) group at the 3rd carbon position and a double bond between the 5th and 6th carbon atoms, distinguishing it from cholesterol. Research suggests it plays a crucial role in the regulation of cholesterol homeostasis and the conversion of cholesterol into bile acids, which are essential for dietary fat digestion and absorption. This metabolic intermediate may also have potential implications in the modulation of lipid metabolism and in conditions such as cholestasis, where bile flow is impaired.

     

    The levels of 3-beta-hydroxy-5-cholestenoate in biological samples can serve as an indicator of bile acid synthesis and cholesterol metabolism, helping researchers understand an individual's metabolic capacity as well as related physiological or pathological states. For example, levels of 3-beta-hydroxy-5-cholestenoate may change in conditions such as liver dysfunction, bile acid synthesis disorders, or under the influence of certain medications. Therefore, measuring 3-beta-hydroxy-5-cholestenoate has potential applications in clinical and research contexts.

     

    1828263999729946624-TheStructureof3-Beta-Hydroxy-5-Cholestenoate.png

    Figure 1. The Structure of 3-Beta-Hydroxy-5-Cholestenoate

     

    MtoZ Biolabs employs an advanced liquid chromatography-mass spectrometry (LC-MS) platform and a skilled metabolomics team to perform in-depth 3-beta-hydroxy-5-cholestenoate analysis service. We assure high sensitivity and precision in the analysis.

     

    Analysis Workflow 

     

    1828264104616906752-Workflowof3-Beta-Hydroxy-5-CholestenoateAnalysisService.PNG

     

    Service Advantages

    1. Advanced Technological Platform

    We use industry-leading mass spectrometry equipment and analysis techniques to ensure high precision and reliability of the analysis results.

     

    2. Extensive Experimental Experience

    With years of experience in experimental operations and data processing, we can handle various complex biological samples.

     

    3. Rapid Data Delivery

    Ensure quick sample processing and timely delivery of research results to facilitate scientific progress.

     

    Applications

    • Qualitative analysis of 3-beta-hydroxy-5-cholestenoate metabolites
    • Quantitative assessment of 3-beta-hydroxy-5-cholestenoate content
    • Baseline for potential enhancements

     

    Sample Submission Requirements

    1. Sample Types

    Serum, plasma, urine, tissues and other biological samples. Kindly submit at least 3 samples of the same type for each test. 

     

    2. Sample Volume

    Minimum 200 μL of liquid sample; Minimum 200 mg of solid tissues.

     

    3. Sample Preservation

    Samples should be stored at -80°C for stability.

     

    Note: Provide details on sample collection and handling.

     

    Deliverables

    1. Experimental Procedures

    2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

    3. Comprehensive Information on 3-Beta-Hydroxy-5-Cholestenoate

    4. Raw Data

    5. Custom Analysis Report

     

    With our 3-beta-hydroxy-5-cholestenoate analysis service, we strive to accelerate your research and product development by delivering precise and reliable results.

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