10x Genomics DNA Sequencing Service
- DNA Purity: Samples should have high purity, with an A260/A280 ratio between 1.8 and 2.0.
- DNA Quantity: Each sample should provide at least 1 µg of high-quality DNA to ensure adequate sequencing data.
- Sample Types: This service is suitable for genomic sequencing of a wide range of organisms, including plants, animals, and microorganisms.
10x Genomics DNA sequencing combines the high-throughput sequencing capabilities of the Illumina system with its unique barcode technology, offering an innovative solution for genomic analysis. By fragmenting DNA samples into smaller pieces and attaching unique barcodes, 10x Genomics enables the reconstruction of long genomic sequences, effectively overcoming the challenges faced by traditional short-read sequencing, particularly in complex and repetitive regions.
10x Genomics DNA sequencing is particularly useful for the in-depth analysis of complex genomes, providing more accurate structural variation data than traditional sequencing methods. This makes it an ideal solution for genomics research, clinical diagnostics, and precision medicine. MtoZ Biolabs offers 10x Genomics DNA Sequencing Service that provide comprehensive support, ranging from sample preparation to data analysis, helping clients achieve breakthroughs in genomic research.
Analysis Workflow
1. Sample Preparation
Genomic DNA is first extracted to ensure purity and integrity, then fragmented into appropriately sized pieces using the 10x Genomics Chromium platform.
2. Barcode Labeling
Each DNA fragment is labeled with a unique barcode, allowing fragments from the same chromosome to be reassembled during later analysis.
3. Sequencing
The Illumina sequencing platform performs high-throughput sequencing on barcode-labeled DNA fragments, generating vast amounts of short-read data.
4. Data Analysis
Specialized bioinformatics tools are used to process the sequencing data, including genome assembly, structural variation detection, and other analyses to reconstruct the complete genome.
5. Results Report
A detailed report is provided, including the quality of the genome assembly, structural variations, and analysis of repetitive regions, offering clients in-depth insights into their genomic data.
Elyanow, R. et al. Bioinformatics. 2017.
Figure 1. 10x Genomics DNA Sequencing Workflow Diagram
Service Advantages
· High-precision genome assembly: 10x Genomics DNA Sequencing Servic ensures precise assembly of long fragments, particularly for complex genomic structures and repetitive regions, resolving issues faced by traditional short-read sequencing technologies.
· Structural variation detection: With 10x Genomics, large-scale structural variations, such as deletions, insertions, inversions, and translocations, can be accurately detected and analyzed, making this technology especially useful for cancer research and genetic disease studies.
· High-throughput and efficiency: By leveraging the Illumina sequencing platform, 10x Genomics DNA Sequencing Service enables the rapid generation of large-scale genomic data, meeting the high-throughput demands of modern research.
· Adaptability to complex genomes: 10x Genomics DNA Sequencing Service provides precision tools for analyzing genomes with abundant repetitive sequences and complex structures, offering solutions to challenges that traditional methods struggle to address.
Sample Submission Suggestions
For optimal sequencing performance, sample quality is critical. The following guidelines are recommended for 10x Genomics DNA Sequencing Service samples:
If the sample quality is below the recommended standards, sequencing results may be compromised. We encourage clients to consult with our technical team prior to sample submission.
Case Study
Case 1: Genome Recombination Mapping with 10x Genomics DNA Sequencing
In this study, 10x Genomics DNA sequencing was combined with linked-read technology to construct a comprehensive genome-wide recombination map. High-precision sequencing of a single individual's genome revealed recombination hotspots and suppression regions within chromosomes. This method provided a high-resolution view of recombination rates across the genome, offering valuable tools for genomic structure research, genetic diversity studies, and breeding in crops and animals. This case highlights the powerful application of 10x Genomics DNA Sequencing Service in genome structure analysis and genetic research, especially in recombination map construction.
Dréau, A. et al. Nat. Commun. 2019.
Case 2: Olive Fly Genome Research Using 10x Genomics DNA Sequencing
This study utilized 10x Genomics DNA sequencing alongside short-read and long-read technologies to assemble high-quality genomes of male and female olive flies. Through linked-read technology, researchers obtained a continuous genome of 489Mb with a scaffold N50 of 4.69Mb, providing essential data for olive fly biological research. The genome assembly also offered new insights into Y chromosome research and advanced insect sterility technology. This case showcases 10x Genomics DNA Sequencing Service's strength in complex genome assembly, particularly in agricultural pest research.
Bayega, A. et al. BMC Genomics. 2020.
10x Genomics DNA sequencing technology offers broad applications in genome assembly, structural variation analysis, and precision medicine. With its ability to address challenges in complex genomes and identify large-scale structural variations, it provides a powerful solution for genomic research. MtoZ Biolabs' 10x Genomics DNA Sequencing Service deliver accurate and reliable genomic data, supporting clients in cancer research, genetic disease diagnosis, plant breeding, and microbial genome studies, thereby advancing scientific research and clinical applications. Contact us for more information!
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