Specificity Evaluation Service for Exosome Product

Exosomes are nanoscale vesicles actively secreted by various types of cells, typically ranging from 30 to 150 nm in diameter, and are widely present in body fluids such as blood, urine, and saliva. Specificity evaluation primarily focuses on assessing the target specificity and purity of components within exosome samples, aiming to verify the compositional integrity, source specificity, and functional relevance of exosome products. This service utilizes a variety of molecular and characterization techniques to identify specific markers within exosomes and analyze their ability to distinguish from non-exosomal components, thereby confirming product specificity and biological consistency.

 

The specificity evaluation service for exosome product is widely applied in the development of exosome-based drug delivery systems, quality control of cell therapy products, biomarker discovery, basic mechanistic research, and regenerative medicine. By precisely evaluating the specificity of exosome products, this service effectively supports quality control in clinical translation, industrialization, and regulatory compliance, providing reliable data to ensure the success of downstream applications.

 



Nag, S. et al. ACS Biomaterials Science & Engineering, 2023.

Figure 1. Exosomes Biogenesis and its Component.

 

Services at MtoZ Biolabs

Based on advanced instruments such as Western blot, flow cytometry (FACS), enzyme-linked immunosorbent assay (ELISA), and high-resolution mass spectrometry (LC-MS/MS), the specificity evaluation service for exosome product launched by MtoZ Biolabs enables in-depth analysis of critical indicators during exosome production, with a focus on accurate detection of exosome-specific markers. The service includes identification of exosome-specific protein markers (e.g., CD63, CD81, TSG101), detection of non-exosomal components, and verification of exosome purity and origin. Through these analyses, the service ensures the acquisition of highly specific, high-purity, and reproducible exosome product data, providing reliable quality assurance for downstream applications.

 

Service Advantages

1. Multi-Platform Integration Ensures Reliable Data

By combining various analytical platforms such as Western blot, FACS, ELISA, and LC-MS/MS, the service provides comprehensive characterization of exosome features, ensuring the accuracy and consistency of the data.

 

2. Broad Coverage of Highly Specific Markers

From classical exosomal markers to source-specific proteins, the service enables precise determination of exosome purity and origin in the sample, minimizing misidentification and enhancing sample reliability.

 

3. High Reproducibility

Standardized operating procedures and quality control systems are employed to ensure high consistency of data across experiments and batches, making the service suitable for process development and batch evaluation.

 

4. Flexible and Customizable Service

Supports both standalone tests and combined analyses. Analytical depth and marker selection can be customized based on different experimental goals, meeting diverse research needs.

 

Applications

1. Drug Delivery Vehicle Validation

In the development of engineered exosomes for drug delivery, it is necessary to confirm whether they express specific targeting molecules or modified proteins. The specificity evaluation service for exosome product can be used to verify marker expression and assess targeting capability.

 

2. Cell-of-Origin Tracing Analysis

By detecting surface or internal markers of exosomes, this service supports the identification of the originating cell type. It is suitable for exosome typing or tissue-specific studies in samples derived from mixed cell sources.

 

3. Disease Biomarker Research

The specificity evaluation service for exosome product can be used to investigate exosomal marker molecules under specific disease conditions for early screening or therapeutic efficacy assessment.

 

4. Functional Study Support and Verification

Before conducting exosome functional experiments (e.g., immune modulation, signal transduction), the specificity evaluation service can confirm the biological attributes of the sample, thereby enhancing the reliability of subsequent research data.

 

FAQ

Q1: How Should Samples Be Submitted? Are there Any Specific Requirements?

A1: We recommend submitting pre-isolated exosome samples. If not feasible, we also accept starting materials such as conditioned media or plasma. We will provide standardized sample preparation guidelines. Samples should be sterile, free of freeze-thaw cycles, and we suggest discussing sample type and quantity with us in advance.

 

Q2: Can the Specificity Evaluation Be Customized Based on my Exosome Modification Strategy?

A2: Yes. We support targeted analysis of specific tags or tissue/disease-specific markers to evaluate the expression and targeting capability of engineered exosomes.

 

Q3: Does the Service Support Parallel Testing of Multiple Samples?

A3: Yes. We have batch processing capabilities and can conduct parallel testing and comparative analysis of multiple samples, making the service suitable for project development, quality control, or process optimization.