Resources

Proteomics Databases



Metabolomics Databases

• • How to Prepare Protein Samples for SDS-PAGE?

  Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a fundamental technique used to separate proteins based on their molecular weight. This paper outlines the detailed steps required for the preparation of protein samples for SDS-PAGE, emphasizing the importance of each step in ensuring accurate and reproducible results.

• • A Step-By-Step Guide to Developing a Sample Preparation Method

  Sample preparation is a critical step in analyzing and studying biological specimens. Whether you're a novice researcher or an experienced scientist, developing a robust sample preparation method can significantly impact the accuracy and reliability of your results. This guide provides a clear, step-by-step approach to developing an effective sample preparation method.

• • What Is the Difference Between Genome and Proteom?

  In the field of biological research, understanding the distinct roles and characteristics of the genome and the proteome is essential. These two concepts, though interrelated, represent different layers of biological information, each contributing uniquely to the understanding of life processes.

• • What Is the Meaning of Proteome?

  In the realm of biological research, the term "proteome" has become increasingly significant, especially in the context of multi-omics approaches and mass spectrometry detection. Derived from the words "protein" and "genome," the proteome represents the entire set of proteins expressed by a genome, cell, tissue, or organism at a given time. Understanding the proteome is crucial for comprehending cellular processes, disease mechanisms, and the development of therapeutic strategies.

• • What Is the Aim of Proteomics?

  Proteomics, the large-scale study of proteins, aims to uncover the complexities of the protein universe, driving advancements in biological research and practical applications. This paper explores the fundamental goals of proteomics, including protein identification, quantification, functional analysis, and clinical applications, highlighting their significance and future directions.

• • What Is Genomics vs Proteomics?

  In the realm of modern biology, genomics and proteomics stand out as two pivotal fields. Both play critical roles in understanding the complexities of life at the molecular level. Genomics focuses on the study of an organism's complete set of DNA, including all of its genes. Proteomics, on the other hand, delves into the entire complement of proteins expressed by a genome. This article aims to elucidate the fundamental differences and interconnections between genomics and proteomics.

• • Advantages and Disadvantages of 2D Blue Native/SDS-PAGE Protein

  2D Blue Native/SDS-PAGE (2D BN/SDS-PAGE) is a widely used technique for studying protein complexes. This method combines the strengths of Blue Native Polyacrylamide Gel Electrophoresis (Blue Native PAGE) and SDS-PAGE, providing detailed information on the assembly status and components of protein complexes. The following section discusses the advantages and disadvantages of this technique in detail.

• • Principle of 2D Blue Native/SDS-PAGE Protein Complex Analysis

  In biochemical and molecular biological research, analyzing protein complexes is crucial for understanding many physiological and pathological processes in organisms. 2D Blue Native/SDS-PAGE (Two-Dimensional Blue Native/SDS-Polyacrylamide Gel Electrophoresis) is a powerful technique for separating and analyzing protein complexes. This article will detail the principles of 2D Blue Native/SDS-PAGE complex analysis.

• • Mechanism of 2D Gel Electrophoresis Image Analysis

  Two-dimensional electrophoresis (2-DE) is a crucial technique in proteomics research used for separating and analyzing complex protein mixtures. It combines isoelectric focusing (IEF) and SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) to separate protein samples, followed by image analysis for quantitative and qualitative assessment of the separation results. This article details the mechanism of two-dimensional electrophoresis image analysis.

• • Workflow of 2D Gel Electrophoresis Image Analysis

  Two-dimensional gel electrophoresis (2-DE) is a high-resolution protein separation technique widely used in proteomics research. It combines isoelectric focusing (IEF) and SDS-PAGE electrophoresis, allowing the separation of complex protein mixtures in two-dimensional space. The analysis of 2-DE images is a crucial part of this technique, enabling quantitative and qualitative understanding of protein expression in samples. The following is the workflow of 2-DE image analysis.