Resources

Proteomics Databases



Metabolomics Databases

• • Mass Spectrometry Detection IP Sample Submission

  Introduction to Sample Amount in Mass Spectrometry for IP Samples: Choosing the appropriate sample amount for mass spectrometry analysis of immunoprecipitation (IP) samples is crucial for ensuring experimental accuracy and effectiveness. Here, we provide essential information on selecting the appropriate sample amounts.

• • IPMS Screens for Unknown Interacting Proteins

  Proteins are fundamental to biological processes, often realizing their functions through interactions with other proteins. Hence, identifying protein-protein interactions is crucial for understanding the complex biological activities within organisms. This paper employs Immunoprecipitation Mass Spectrometry (IPMS) to explore unknown protein interactions.

• • High-Throughput Tandem Mass Spectrometry Protein Identification Technology

  With advancements in technology, protein research has transitioned from analyzing single proteins to conducting quantitative analyses of entire proteomes. This progression necessitates the use of high-throughput tandem mass spectrometry for protein identification, a highly efficient technique based on mass spectrometry that facilitates the rapid and precise identification of proteins within the proteome.

• • Overview of N-Terminal Protein Sequencing

  N-terminal protein sequencing is used to determine the n-terminal amino acid sequence of protein molecules. The n-terminus, or amino terminus, is the starting point of a protein chain and usually plays an important role in protein folding and function. In n-terminal sequencing, Edman degradation or mass spectrometry is usually used. Edman degradation method is a traditional n-terminal sequencing method, which gradually removes one amino acid from the n-terminal by chemical reaction and id......

• • Maldi Tof Technique

  Maldi Tof technique employed for the mass spectrometric analysis of biomolecules and macromolecular compounds. This technique uses laser energy to achieve desorption and ionization of sample molecules, followed by mass analysis via a time-of-flight spectrometer. Due to its efficiency, rapidity, and high sensitivity, MALDI TOF has found extensive applications in biomedicine, proteomics, microbial identification, and drug development. The key mechanism involves the absorption and transmission of laser e......

• • Affinity Proteomics

  Affinity proteomics is a technique leveraging specific interactions to extract and analyze proteins from complex biological samples. It utilizes specific ligands or antibodies to capture target proteins and their interacting partners, identifying protein complex components and elucidating their biological functions. This method finds wide applications in life sciences, including signal transduction, metabolic pathways, and disease mechanisms. By examining interactions among proteins, researchers can m......

• • Tandem Affinity Purification Mass Spectrometry

  Tandem Affinity Purification Mass Spectrometry (TAP-MS) is an advanced technique that integrates protein purification with mass spectrometry analysis, extensively employed in cell biology, molecular biology, and biochemistry. This method focuses on identifying and quantifying proteins and protein complexes through specific affinity purification steps coupled with mass spectrometry. TAP-MS allows researchers to isolate target proteins from complex biological samples, facilitating accurate qualitative a......

• • Protein Expression Studies

  Protein Expression Studies are critical area of study in biology, focusing on the processes of synthesis, folding, modification, and degradation of proteins within organisms. Proteins are essential to life, facilitating cellular functions, signal transduction, and metabolic regulation. By studying protein expression, scientists can better understand genomic functions and elucidate how genetic mutations contribute to diseases. This research not only unveils fundamental life principles but also underpin......

• • Pea Protein Complete Amino Acid Profile

  Pea protein complete amino acid profile is a method used to determine the amino acid composition of pea protein. This process can reveal the nutritional value of pea protein in detail and provide valuable information about its functional properties in different applications. Pea protein is an important source of plant protein, attracting attention for its rich nutritional components and excellent digestibility. Through pea protein complete amino acid analysis, scientists and producers can obtain compr......

• • Mass Spectrometry Peptide Fragmentation

  Mass spectrometry peptide fragmentation is an essential technique in proteomics, primarily used to discern the primary structure of peptides. This method involves three main steps: peptide ionization, selection, and fragmentation. Initially, peptides are ionized to form charged ions, which are then selected for analysis using the mass spectrometer's mass selection capabilities. Subsequently, these ions are fragmented into smaller components via techniques such as collision-induced dissociation (CID), ......