Resources
Proteomics Databases
Metabolomics Databases
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• Edman Degradation Sequencing
Edman degradation sequencing is a classic method used to determine the primary structure of proteins. This technique chemically modifies the N-terminal amino acid of a protein, enabling its stepwise removal from the N-terminus. This process generates a series of identifiable derivatives, facilitating the analysis of protein sequences. Specifically, the method involves the reaction of an isothiocyanate acyl derivative with the N-terminal amino acid of the polypeptide chain, producing a phenylthiohydant......
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Protein Quality Assessment is a critical technique in modern biological and medical research, designed to evaluate the purity, integrity, functionality, and biocompatibility of protein samples. This process is essential for ensuring that protein components in biologics, drug development, and the food industry meet the required standards. Protein quality assessment is not limited to biopharmaceutical applications but is also widely used in food safety testing, nutritional research, environmental monito......
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Top-down mass spectrometry is a technique used in proteomics to analyze intact proteins directly, without the need to cleave them into peptides, as in the traditional "bottom-up" approach. This method significantly enhances the resolution and accuracy of proteomic studies, especially in the investigation of post-translational modifications, polymorphisms, and isoforms of proteins. Top-down mass spectrometry provides detailed structural information about proteins, including the precise locations and ty......
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• Gas Chromatography Mass Spectrometry Analysis
Gas Chromatography Mass Spectrometry analysis is a powerful analytical technique that combines gas chromatography and mass spectrometry, widely used in the fields of chemistry and biology for the separation and identification of complex mixtures. During the gas chromatography phase, compounds in the sample are separated based on their differing distribution coefficients between the gas mobile phase and the stationary phase. These separated compounds then enter the mass spectrometer, where they are io......
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LC-MS/MS analysis combines the strengths of liquid chromatography (LC) and mass spectrometry (MS), enabling the efficient separation and precise analysis of complex samples. In this analysis, LC is used to separate the various components of a sample, while tandem mass spectrometry (MS/MS) detects and identifies these separated components. In LC, compounds are separated based on their chemical properties, such as polarity, size, or molecular structure, through interactions between the mobile and stati......
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Epitope Mapping is a technique used to characterize the interactions between antibodies and antigens and to identify antigenic epitopes. An antigen epitope is a specific region of a protein that can be recognized and bound by the immune system, typically by antibodies or T cell receptors. Epitope mapping is widely applied in biomedical research for vaccine design, diagnostic tool development, and studying the mechanisms of autoimmune diseases. By identifying antigenic epitopes, researchers can gain a......
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ICP-MS analysis (Inductively Coupled Plasma Mass Spectrometry) is a high-sensitivity, high-resolution analytical technique extensively used for elemental analysis, trace element detection, and isotope ratio analysis. This technology combines inductively coupled plasma (ICP) with mass spectrometry (MS), allowing precise detection of elements and their isotopic compositions in samples at extremely low concentrations. As a powerful multi-element analytical tool, ICP-MS has been widely applied across var......
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Ion Chromatography-Mass Spectrometry (IC-MS) integrates ion chromatography (IC) with mass spectrometry (MS) and is widely applied across fields such as chemistry, biology, environmental science, and food safety. Ion chromatography is a technique used to separate ionic compounds, allowing for the efficient detection of both cations and anions in complex samples. Mass spectrometry, meanwhile, analyzes molecular mass and structure by ionizing the sample and measuring its mass-to-charge ratio (m/z), prov......
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DSC and TGA analysis are widely used thermal characterization techniques in fields such as materials science, biology, and chemistry. DSC is primarily employed to assess the thermal properties of materials by recording the heat flow difference between the sample and the reference during temperature changes. This technique is used to investigate thermal behaviors such as phase transitions, glass transitions, crystallization, and melting. TGA, on the other hand, measures the relationship between sample......
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• De Novo Sequencing in Proteomics Research
De novo sequencing, a method widely used in proteomics research, allows for the determination of the amino acid sequence of unknown or partially known proteins. This technique is essential in scientific exploration and biomedical research because it overcomes the limitations of traditional database-dependent methods, enabling the direct derivation of protein sequences from raw mass spectrometry data. De novo sequencing is particularly useful for studying new species, phenotypic variations, or genomic......
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