Edman Sequencing Service in Protein Analysis
Edman sequencing service is a specialized analytical technique used for determining the N-terminal amino acid sequence of proteins with high precision. Named after its inventor, Pehr Edman, this method employs Edman degradation to sequentially cleave amino acids from the N-terminus of a peptide, enabling their identification and quantification. This approach remains one of the most reliable tools for analyzing short peptide sequences, offering valuable insights into protein structure and functional characteristics.
In biomedical research, Edman sequencing service plays a pivotal role in protein function characterization, protein engineering, and the development of biopharmaceuticals. Researchers use this technique to analyze unknown protein sequences, predict their biological roles, and compare them with known protein structures. Although primarily used for sequence analysis, Edman sequencing service also supports studies on protein processing pathways and regulation mechanisms.
In clinical applications, Edman sequencing service contributes to identifying disease-related protein variants, providing foundational data for diagnostic biomarker discovery and therapeutic strategy development. Additionally, this technology aids in drug target identification and optimization, ensuring both drug efficacy and safety throughout the development pipeline.
Analysis Workflow of Edman Sequencing Service
1. Sample Preparation
Proteins are purified, concentrated, and cleaved into peptide fragments compatible with Edman degradation.
2. Edman Degradation Reaction
The N-terminal amino acid reacts with PITC, forming a stable derivative analyzed by HPLC.
3. Amino Acid Identification
Sequential analysis enables precise amino acid determination until the sequence is fully resolved.
Advantages and Challenges of Edman Sequencing Service
1. Advantages
High accuracy, effective for short peptides, and independent of reference databases, making it ideal for novel protein studies.
2. Challenges
Requires high sample purity, is time-consuming for long peptides, and cannot process N-terminally blocked proteins.
At MtoZ Biolabs, our Edman sequencing service delivers high-quality sequence data tailored to diverse research needs. With expertise in sequencing technology and a commitment to scientific rigor, we support researchers and biopharmaceutical enterprises in achieving precise and impactful discoveries.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
How to order?
