1-Methyl-L-Histidine Analysis Service

1-Methyl-L-Histidine is an important amino acid derivative formed by the methylation of histidine. It plays a crucial role in protein synthesis and muscle metabolism, being one of the most prevalent histidine derivatives in muscles. Its metabolic pathways include release during protein degradation and functioning as a signaling molecule in various physiological processes. Given its significance in exercise metabolism, nutritional assessment, and disease monitoring, precise analysis of 1-Methyl-L-Histidine levels and metabolism is essential for understanding its functions in the body.

 



Figure 1. Molecular Structure of 1-Methyl-L-Histidine

 

MtoZ Biolabs utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) to conduct detailed quantitative and qualitative analyses of 1-Methyl-L-Histidine. This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of 1-Methyl-L-Histidine in complex biological matrices.

 

Analysis Workflow

 

 

 

Service Advantages

 



 

Sample Submission Requirements

Sample Types

Serum, plasma, urine, tissue homogenates, and other biological samples.

 

Sample Volume

At least 200 μL of liquid sample or 200 mg of tissue sample.

 

Sample Preservation

Store and transport samples at -80°C to maintain 1-Methyl-L-Histidine stability.

 

Applications

Clinical Research: Monitor 1-Methyl-L-Histidine levels to study its roles in metabolic diseases, immune function, and neurological disorders.

 

Disease Monitoring: Analyze 1-Methyl-L-Histidine and its metabolites to monitor disease progression, such as muscle diseases and malnutrition.

 

Nutritional Research: Evaluate the 1-Methyl-L-Histidine content in foods and supplements to guide nutritional intake and health management.

 

Drug Development: Explore the potential applications of 1-Methyl-L-Histidine and its metabolites in new drug development, assessing drug efficacy and safety.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 1-Methyl-L-Histidine

4. Raw Data

5. Custom Analysis Report