Mammalian Plasma Proteome Quantitative Analysis Service
Mammalian plasma proteomes are among the most valuable research targets in biomedical research and translational applications. Plasma contains a wide variety of proteins that play essential roles in maintaining homeostasis and regulating metabolic pathways, while also being closely associated with disease onset, progression, and treatment response. Quantitative analysis of plasma proteomes can reveal disease biomarkers, drug action mechanisms, and metabolic dynamics, providing crucial support for early diagnosis, therapeutic evaluation, and personalized medicine.
Núñez, E. et al. Biomedicines. 2024.
Figure 1. Human Plasma Proteome Quantitative Analysis Workflow
Leveraging advanced high-resolution mass spectrometry platforms and optimized sample preparation workflows, MtoZ Biolabs offers the mammalian plasma proteome quantitative analysis service that enables precise characterization of protein abundance and modifications. By elucidating dynamic changes within plasma proteomes, the service facilitates biomarker discovery, supports drug development, and advances both fundamental research and clinical translation, providing researchers and pharmaceutical developers with systematic, standardized, and reproducible plasma protein quantification.
Services at MtoZ Biolabs
Our mammalian plasma proteome quantitative analysis service is performed using liquid chromatography–tandem mass spectrometry (LC-MS/MS). By integrating both targeted and untargeted approaches, this service enables precise quantification and functional interpretation of complex protein populations in plasma. MtoZ Biolabs offers the following services in this area:
✔️Proteome profiling
Constructing global expression maps of plasma proteins to reflect overall biological states.
✔️Relative quantification and differential analysis
Comparing protein expression differences between groups using label-based strategies (TMT/iTRAQ/SILAC) or label-free approaches to identify potential biomarkers.
✔️Absolute quantification
Measuring absolute protein concentrations using internal standards or calibration curves.
✔️Post-translational modification detection
Quantifying modifications such as phosphorylation, glycosylation, and acetylation to reveal their regulatory roles.
✔️Pathway and network analysis
Integrating bioinformatics tools for pathway enrichment, GO annotation, and interaction network construction.
Beyond data acquisition, MtoZ Biolabs provides end-to-end solutions that include experimental design, sample preparation, mass spectrometry detection, data analysis, and interpretation, ensuring results that are directly applicable to research and development.
Analysis Workflow
1. Sample preparation: Plasma preprocessing and depletion of high-abundance proteins.
2. Protein digestion: Enzymatic digestion (e.g., trypsin) to generate peptides.
3. Mass spectrometry detection: LC-MS/MS analysis with high-resolution data acquisition.
4. Data processing: Database searching, quantification, and differential protein screening.
5. Bioinformatics interpretation: Functional annotation, pathway enrichment, and network construction.
6. Result delivery: Comprehensive technical reports with visualized data outputs.
Service Advantages
1. High-Resolution and High-Sensitivity Platform
MtoZ Biolabs employs Thermo Orbitrap mass spectrometers coupled with nano-LC systems, enabling deep coverage and highly sensitive detection of plasma proteins. This setup allows for reliable quantification of low-abundance proteins while minimizing background interference.
2. Standardized Sample Preparation
Plasma contains high-abundance proteins (such as albumin and immunoglobulins) that can mask signals from low-abundance proteins. Our optimized depletion strategies and standardized SOPs ensure reproducibility, stability, and improved downstream detection.
3. Flexible Analytical Strategies
We support multiple quantification modes, including DDA, DIA, PRM/MRM, as well as label-based approaches (such as TMT/iTRAQ) and label-free quantification methods. This flexibility allows tailored analytical strategies according to project goals, ensuring suitability for both large-scale discovery and targeted validation studies.
4. Customized Services
MtoZ Biolabs designs a customized mammalian plasma proteome quantitative analysis service according to client research objectives, offering full support from project planning to data interpretation. This ensures that final results are closely aligned with the intended research goals.
5. One-Time-Charge
Our pricing is transparent, with no hidden fees or additional costs.
Applications
1. Biomarker discovery: Identification of potential diagnostic and prognostic markers in plasma.
2. Drug mechanism studies: Profiling protein changes before and after therapeutic intervention.
3. Personalized medicine: Characterization of patient-specific protein expression patterns.
4. Fundamental biological research: Investigating the role of proteins in physiological and pathological processes.
FAQs
Q1: How much sample is required?
A1: Typically, tens to hundreds of microliters of plasma are required, depending on the project design. Detailed sample submission guidelines are provided.
Q2: What is the turnaround time?
A2: Results are usually delivered within several weeks after sample receipt, depending on the number of samples and analysis depth.
Sample Submission Suggestions
1. Sample type: Mammalian plasma.
2. Storage condition: Store at -80°C, avoid repeated freeze-thaw cycles.
3. Transport: Ship on dry ice to ensure sample stability.
4. Preparation notes: Inform us in advance if special preprocessing is required.
5. Submission guide: MtoZ Biolabs provides detailed sample submission instructions.
Deliverables
1. Detailed description of experimental steps and methods
2. Mass spectrometry parameters and spectra
3. Raw data files (RAW/mzML format)
4. Protein quantification result tables
5. Differential protein analysis and statistics
6. Functional annotation and pathway analysis charts
7. Comprehensive interpretation with visualized data presentation
Quantitative analysis of mammalian plasma proteome is a vital tool for understanding disease mechanisms, discovering biomarkers, and advancing drug development. With high-resolution mass spectrometry platforms, standardized workflows, and comprehensive data interpretation, MtoZ Biolabs delivers reliable, end-to-end mammalian plasma proteome quantitative analysis service for both academic and industrial clients.
If you are seeking a professional partner for plasma proteome quantification, contact MtoZ Biolabs today. We are committed to providing high-quality technical support and data outputs to accelerate your scientific and development goals.
Related Services
Quantitative Proteomics Service
Label-Free Quantitative Proteomics Service, MS Based
iTRAQ/TMT/MultiNotch Quantitative Proteomics Service
SILAC/Dimethyl Quantitative Proteomics Service
How to order?
