TMT Quantitative Proteomics Analysis Service, Assisting Cutting-Edge Scientific Research

MtoZ Biolabs offers TMT quantitative proteomics analysis service powered by the advanced Orbitrap Fusion Lumos mass spectrometer and Nano-LC system, ensuring high-resolution, high-sensitivity protein identification and quantification. This service employs a dual fragmentation strategy (HCD and ETD) to achieve comprehensive detection of low-abundance proteins while delivering reliable, high-quality data. By integrating state-of-the-art technology and expert workflows, our TMT-based approach provides a robust platform for life sciences research.

 

TMT (Tandem Mass Tags) quantitative proteomics analysis is an isotope-based labeling technology designed for high-throughput protein analysis. The method involves the covalent attachment of TMT tags with distinct isotopic masses to peptides in each sample through a chemical reaction. Samples labeled with different isotopes are pooled, fractionated using high-performance liquid chromatography (HPLC), and subsequently analyzed by tandem mass spectrometry (MS/MS). This approach enables the simultaneous relative quantification of up to 18 samples, making it a powerful tool for large-scale proteomic studies.

 

Workflow of TMT Quantitative Proteomics Analysis Service

MtoZ Biolabs offers a meticulously designed TMT quantitative proteomics analysis service that spans from sample preparation to data interpretation, ensuring precise and reproducible protein quantification. The workflow is detailed below:

 

1. Sample Preparation

MtoZ Biolabs employs a systematic protein preparation workflow tailored to sample types, incorporating denaturation, reduction, and alkylation steps to maximize the exposure of reactive functional groups. Optimized enzymatic digestion protocols are then applied to generate high-quality peptide mixtures, forming a reliable basis for downstream TMT labeling and comprehensive proteomic analysis.

 

2. TMT Labeling

We use high-performance TMT reagents to label enzymatically digested peptides. These labeled peptides are pooled into a composite sample, enabling streamlined chromatographic separation and mass spectrometry analysis. This step ensures experimental reproducibility and high throughput for large-scale studies.

 

3. Peptide Fractionation

HPLC fractionates complex peptide mixtures, significantly improving peptide resolution and enhancing proteome coverage. Our fractionation protocols ensure both depth and precision in protein expression profiling.

 

4. Mass Spectrometry Analysis

Utilizing the Thermo Fisher Orbitrap Fusion Lumos mass spectrometer, integrated with Nano-LC, MtoZ Biolabs delivers high-resolution and high-sensitivity analysis of complex protein samples. This ensures accurate protein identification and precise quantification, even for low-abundance proteins.

 

5. Data Processing and Quantitative Analysis

MS/MS data are analyzed using advanced computational tools, with relative protein abundances determined based on the intensities of reporter ions. Comprehensive database searches further ensure reliable protein identification and quantification, facilitating robust and reproducible outcomes in TMT quantitative proteomics analysis service.

 

Applications

1. Disease Mechanism Research

TMT quantitative proteomics analysis service enables the sensitive identification and quantification of disease-associated protein expression changes, providing essential insights into the molecular mechanisms underlying disease initiation and progression.

 

2. Drug Target Discovery and Validation

By quantifying protein expression alterations in response to drug treatments, MtoZ Biolabs identifies potential therapeutic targets and evaluates the regulatory impact of drugs on specific protein networks and biological pathways.

 

3. Biomarker Screening

Using high-throughput TMT proteomics, we identify critical protein biomarkers for diagnosis, prognosis, and therapy evaluation, driving advancements in precision medicine and personalized treatments.

 

Why Choose MtoZ Biolabs?

Our primary advantages include:

1. Advanced Analysis Platform

MtoZ Biolabs' advanced TMT quantitative proteomics analysis platform guarantees reliable, fast, and highly accurate analysis service.

 

2. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

3. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all TMT quantitative proteomics analysis data, providing clients with a comprehensive data report.

 

What Could Be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

5. Bioinformatics Analysis

6. Raw Data Files