4D Label-Free Quantitative Proteomics Service

4D Label-Free quantitative proteomics is an innovative label-free quantitative proteomics technology utilizing the timsTOF Pro ion mobility platform. This technology employs a data-dependent acquisition-synchronous accumulation of continuous fragmentation (ddaPASEF) scanning mode, which enhances sensitivity, throughput, and coverage depth in proteomics analysis. By integrating data-dependent acquisition (DDA) with synchronous accumulation of continuous fragmentation (PASEF), the 4D technology improves ion mobility separation based on the collision cross-section (CCS) of peptides, thereby enhancing ion utilization and accuracy. MtoZ Biolabs is dedicated to utilizing this cutting-edge technology to support biomedical research, particularly in molecular biology.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity

4D technology enables detection of trace amounts, making it ideal for analyzing low-abundance proteins.

 

2. High Throughput

The rapid scanning speeds significantly boost sample analysis efficiency.

 

3. Rich Information

Offers extensive insights into protein expression and variability, facilitating the discovery of biomarkers and drug targets.

 

4. Label-Free Benefit

Eliminates interference from labeling agents, offering greater adaptability and convenience.

 

Sample Submission Requirements

 

 

For more sample details, please consult our technical team.

 

Applications

• Analysis of protein expression profiles in drug responses and gene knockout/overexpression studies.
• Physiological investigations into plant and microbial growth and responses to stress.
• High-throughput analysis of disease biomarkers and drug action targets.
• Screening for mechanisms of protein action and specialized functional proteins.
• Development of proteomic maps across various species.
• Suitable for clinical analysis of trace samples, such as exosomes, FFPE tissue sections, and biopsy materials.

 

Application Case

Investigation of the bactericidal mechanism of Penicilazaphilone C on Escherichia coli based on 4D label-free quantitative proteomic analysis

 

 

Zhao, H. G. et al. Eur J Pharm Sci. 2022.

 

Our 4D Label-Free Quantitative Proteomics Service provides high-quality data support, advancing your scientific research and applications. For any inquiries or requirements, please contact us.