Tandem Mass Tag Proteomics Service
Tandem Mass Tag proteomics is a sophisticated quantitative mass spectrometry (MS) technique designed to enable the simultaneous identification and quantification of proteins across multiple biological samples within a single experiment. Tandem Mass Tag proteomics approach facilitates high-throughput proteomic analyses with enhanced precision and accuracy, making the technical a powerful tool for comparative studies in various biological contexts. To support your advanced research needs, MtoZ Biolabs offers our Tandem Mass Tag Proteomics Service, delivering reliable and comprehensive protein quantification solutions.
Each Tandem Mss Tag (TMT) reagent used in Tandem Mass Tag Proteomics consists of three key components: a reporter ion, a mass-normalization group, and a reactive group. The reporter ion provides the quantitative signal unique to each sample. The mass-normalization group ensures that all TMT tags are isobaric, maintaining identical mass across different tags despite variations in the reporter region. The reactive group, commonly an NHS-ester, covalently binds to primary amines on peptides, facilitating stable attachment during the proteolytic digestion process. Upon fragmentation in the mass spectrometer, the reporter ions are released and detected, enabling precise relative quantification of peptides and, consequently, proteins across the multiplexed samples.
Figure 1. The Chemical Structure of Tandem Mass Tag (TMT) Reagents
One of the standout features of Tandem Mass Tag Proteomics technology is that it allows up to 16 different samples to be labeled and analyzed concurrently in a single MS run due to its high multiplexing capability. This multiplexing is achieved by using TMT reagents with distinct reporter ions that can be resolved in the MS/MS spectra. By labeling each sample with a unique TMT tag, researchers can minimize technical variability and enhance throughput, as all samples are processed and measured under identical experimental conditions. This simultaneous analysis not only increases efficiency but also improves the accuracy of comparative studies, making Tandem Mass Tag Proteomics an invaluable tool for large-scale proteomic investigations.
Analysis Workflow
1. Sample Preparation
2. TMT Labeling Process
3. Peptide Fractionation
4. Mass Spectrometry Analysis
5. Data Acquisition and Analysis
Hou, J. et al. Front Cardiovasc Med. 2022.
Figure 2. Workflow for Tandem Mass Tag Proteomics Service
Service Advantages
1. Advance Analysis Platform: MtoZ Biolabs established an advanced Tandem Mass Tag Proteomics Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. High Multiplexing Capacity: Our TMT technology allows for the labeling and concurrent analysis of up to 16 distinct samples in a single mass spectrometry (MS) run. This high level of multiplexing significantly increases throughput and efficiency, enabling large-scale studies without compromising data quality.
3. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
4. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all tandem mass tag proteomics data providing clients with a comprehensive data report.
5. Fast Turnaround: Efficient workflows and dedicated resources enable us to deliver timely results without compromising quality.
Applications
1. Biomarker Discovery
Tandem Mass Tag Proteomics Service plays an essential role in the discovery of biomarkers for disease detection, prognosis, and monitoring therapeutic responses. By analyzing multiple samples in parallel, it enables the identification of protein signatures that can serve as reliable biomarkers in clinical diagnostics.
2. Clinical Research
In clinical research, tandem mass tag proteomics is applied to understand the underlying mechanisms of various diseases, including cancer, neurodegenerative disorders, and metabolic diseases. This technique helps identify disease-specific biomarkers and provides insights into how diseases alter protein profiles, offering potential pathways for early diagnosis and treatment.
3. Drug Discovery
Tandem Mass Tag Proteomics Service is crucial in drug discovery, allowing researchers to identify novel biomarkers and target proteins involved in disease progression. By quantifying protein expression and post-translational modifications, this service aids in the development of more effective therapeutic strategies.
4. Proteomic Profiling
Tandem Mass Tag Proteomics Service offers high-throughput proteomic profiling of complex biological samples, enabling comprehensive analysis of proteins across a wide range of biological systems. This capability is essential for unraveling intricate biological networks, studying protein-protein interactions, and discovering new therapeutic targets.
Case Study
Tandem Mass Tag Proteomics Profiling Identifies Candidate Serum Biomarkers of Drug-Induced Liver Injury in Humans
Ravindra, K. C. et al. Nat Commun. 2023.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
At MtoZ Biolabs, we are committed to providing high-quality, reliable, and personalized analysis services. If you are interested in our Tandem Mass Tag Proteomics Service, please feel free to contact us.
How to order?
