Quantification Mass Spectrometry Service
- Global Quantification: Using TMT or iTRAQ labeling, suitable for multi-sample comparisons and protein expression level studies.
- Targeted Quantification: Employing SRM/MRM methods to provide high-sensitivity detection of specific proteins.
- Differential Metabolite Screening: Unveiling the dynamic regulation of metabolic pathways.
- Accurate Metabolite Quantification: Supporting biomarker discovery and functional validation.
- Metabolic Pathways and Distribution: Mapping the pathways and distribution of drugs within the body.
- Metal-based Drug Development: Quantifying metal ions and their complexes to support the development of metal-based therapies.
- MALDI-TOF MS: Ideal for fast screening of metabolites and large molecules.
- ESI-MS: Perfect for precise detection of low-molecular-weight compounds.
- LC-MS/MS: Ideal for broad protein and metabolite quantification.
- Orbitrap MS and FTICR-MS: Provide high-resolution analysis for complex samples.
- ICP-MS: Specialized for the detection of metal ions and inorganic molecules.
- Data Normalization and Differential Screening: Ensuring consistency and accuracy in measurements.
- Biological Function Annotation and Pathway Analysis: Translating data into meaningful biological insights.
- Detailed Reports: Providing in-depth data analysis and customized reports for your research needs.
Quantification mass spectrometry is a highly precise and efficient analytical technique used to directly measure the concentration of target molecules in biological samples. With its high sensitivity, broad dynamic range, and high resolution, quantification mass spectrometry is capable of accurately revealing dynamic molecular changes and biological network mechanisms. It is widely applied in proteomics, metabolomics, drug development, disease diagnosis, and environmental sciences.
MtoZ Biolabs offers comprehensive Quantification Mass Spectrometry Service utilizing various cutting-edge platforms (including MALDI-TOF MS, ESI-MS, LC-MS/MS, Orbitrap MS, FTICR-MS, and ICP-MS) to provide researchers with reliable, high-quality data, significantly advancing scientific research.
Services at MtoZ Biolabs
MtoZ Biolabs' Quantification Mass Spectrometry Service provide comprehensive solutions to meet diverse research needs by combining different mass spectrometry techniques to deliver flexible and precise results:
1. Proteomics Quantification
Using advanced LC-MS/MS and Orbitrap MS platforms, we provide both global and targeted protein quantification.
2. Metabolomics Quantification
We use MALDI-TOF MS, FTICR-MS, and LC-MS/MS to quantitatively analyze metabolite concentration variations.
3. Multi-Omics Quantification
By integrating proteomics and metabolomics, we offer a multi-layered view of biological networks, providing valuable insights into complex molecular mechanisms.
4. Drug Development Support
In drug metabolism and pharmacokinetics studies, we utilize ICP-MS and LC-MS/MS platforms to quantify drugs and their metabolites.
5. Environmental and Materials Analysis
For complex matrices (e.g., soil, water, materials), we provide MALDI-TOF MS and ICP-MS for precise target molecule or metal ion detection.
6. Complex Matrix Sample Quantification
Using FTICR-MS and LC-MS/MS, we deliver high-resolution quantification for complex biological samples (serum, plasma, tissues), supporting biomedical research.
Analysis Workflow
Our Quantification Mass Spectrometry Service follow a standardized, efficient workflow combined with customized solutions to provide accurate and precise results for each research project:
1. Sample Preparation
We support a wide range of sample types, including serum, plasma, urine, tissues, cell extracts, and environmental samples. Our team provides detailed sample preparation guidelines to ensure the highest quality and reliability of your data.
2. Mass Spectrometry Detection
We choose the optimal instrument combinations based on the nature of your samples and target molecules:
3. Data Analysis
4. Report Delivery
Our reports include comprehensive data with visual representations, such as volcano plots, heatmaps, and functional pathway diagrams, along with raw mass spectrometry data.
Moghieb, A. et al. Translational Proteomics. 2013.
Figure 1. General workflow for quantitative mass spectrometry-based translational neuroproteomic methods
Why Choose MtoZ Biolabs?
MtoZ Biolabs' Quantification Mass Spectrometry Service stand out due to its cutting-edge technology, excellent customer support, and tailored solutions. We are your trusted partner in advancing scientific research:
· State-of-the-Art Instrumentation: We combine multiple mass spectrometry technologies (including MALDI-TOF MS, ICP-MS, and Orbitrap MS) to meet diverse needs, from high-throughput screening to precise quantification.
· High Sensitivity and Broad Dynamic Range: Capable of detecting concentrations from pg/mL to μg/mL, ensuring precise results even for low-abundance molecules.
· Custom Solutions: We tailor experimental protocols to your specific research goals, ensuring that our results meet your exact needs.
· Rapid Turnaround: With our streamlined processes, results can typically be delivered within weeks under standard protocols.
· One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Case Study
Case 1: Quantification Mass Spectrometry in Alzheimer's Disease Biomarker Discovery
This study used quantification mass spectrometry to analyze cerebrospinal fluid samples from Alzheimer's disease (AD) patients. Through high-precision quantification, the research identified potential protein biomarkers related to AD, offering valuable insights for early diagnosis and understanding disease mechanisms. This study highlights the potential of Quantification Mass Spectrometry Service in disease research, particularly for precision medicine and clinical diagnostics.
Watson, A. I. et al. Sci. Data. 2023.
Case 2: Application of Quantification Mass Spectrometry in Metabolite Analysis
In this study, Gas Chromatography-Mass Spectrometry (GC-MS) was used to simultaneously quantify polar metabolites and carbon isotope distribution. The technique provided high-efficiency, precise quantification, shedding light on dynamic metabolic changes and pathways. This case demonstrates the strength of Quantification Mass Spectrometry Service in metabolomics, offering solid support for in-depth analysis of biological processes and metabolic networks.
Evers, B. et al. Anal. Chem. 2021.
FAQ
Q1: What is the sensitivity of Quantification Mass Spectrometry? Is it suitable for detecting low-abundance molecules?
Quantification mass spectrometry offers extremely high sensitivity, capable of detecting molecules at concentrations as low as pg/mL. Using platforms like Orbitrap MS and LC-MS/MS, combined with isotope labeling (e.g., SILAC) and targeted methods (e.g., SRM/MRM), we can achieve high-precision detection of low-abundance molecules, making it ideal for in-depth studies of proteins and metabolites, even in complex samples.
MtoZ Biolabs is committed to providing high-quality Quantification Mass Spectrometry Services for researchers worldwide, empowering your scientific discoveries and technological innovations. Contact us today for a free consultation and learn how we can support your research!
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
Quantitative Proteomics Service
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