In Vitro Pull-Down Assay Service
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State-of-the-Art Instrumentation: Our facility is equipped with advanced chromatography systems, high-resolution mass spectrometers, and imaging platforms for precise analysis.
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Cutting-Edge Reagents: We use high-quality beads (agarose, magnetic, etc.), cross-linking agents, and validated antibodies to ensure robust and reliable results.
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Bait Molecule Preparation: We assist in the purification or synthesis of bait molecules, such as proteins, peptides, or nucleic acids.
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Immobilization: Bait molecules are immobilized on solid supports, such as agarose or magnetic beads, using covalent or affinity-based methods.
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Incubation with Prey: The immobilized bait is incubated with prey molecules under optimized conditions to allow interaction.
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Wash and Elution: Non-specific binders are removed through stringent washing steps, and specific interactors are eluted.
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Downstream Analysis: Eluted complexes are analyzed using SDS-PAGE, Western blot, or mass spectrometry for identification and quantification of binding partners.
- Elucidating signaling pathways
- Characterizing receptor-ligand interactions
- Validating high-throughput screening results
- Screening for small molecule inhibitors or activators
- Studying target engagement and binding kinetics
- Investigating protein-DNA or protein-RNA interactions
- Mapping epigenetic regulators
- Identifying components of protein complexes
- Analyzing conformational changes upon binding
In vitro pull-down assay is a powerful biochemical technique widely used to investigate molecular interactions under controlled experimental conditions. This assay involves isolating specific proteins or molecular complexes from a mixture through the use of a bait molecule immobilized on a solid support. By mimicking physiological interactions, this technique provides valuable insights into protein-protein, protein-nucleic acid, or protein-small molecule interactions. At MtoZ Biolabs, we provide a comprehensive in vitro pull-down assay service tailored to meet the specific needs of our clients in studying molecular interactions with high precision and reproducibility.
Understanding the fundamental aspects of molecular interactions is critical in life sciences research. Whether studying signaling pathways, identifying novel binding partners, or validating interactions predicted by bioinformatics, pull-down assays serve as a cornerstone in molecular biology and biochemistry. With cutting-edge facilities and an experienced team, MtoZ Biolabs ensures high-quality results that accelerate your research.
Wierer, M. et al. Hum Mol Genet. 2016.
Figure1. In vitro pulldown assays to study protein interactions with modified histone tails, DNA and asembled nucleosomes.
Why Choose In Vitro Pull-Down Assays?
1. Precision and Specificity: In vitro pull-down assays enable precise isolation and identification of interacting molecules. By using purified components in a controlled environment, this technique minimizes the complexity and variability associated with in vivo experiments, allowing researchers to focus on specific interactions.
2. Broad Applications:
① Protein-Protein Interactions: Identify direct physical interactions between proteins.
② Protein-Nucleic Acid Interactions: Study how proteins bind to DNA or RNA molecules.
③Protein-Small Molecule Interactions: Assess binding affinities and interaction dynamics.
3. Versatility: The pull-down assay can be customized to investigate various types of molecular interactions, including those involving fusion proteins, post-translational modifications (PTMs), or multi-protein complexes.
4. Compatibility with Downstream Analyses: Pull-down assay products can be further analyzed using mass spectrometry (MS), Western blotting, or other techniques, enabling detailed characterization of interaction partners.
Advantages of Our In Vitro Pull-Down Assay Service
At MtoZ Biolabs, we provide an end-to-end in vitro pull-down assay service that covers experimental design, assay optimization, execution, and data interpretation. Our service is tailored to address the specific requirements of each project, ensuring accurate and reproducible results.
1. Expertise in Assay Design
Our team of scientists works closely with clients to design customized pull-down assays. We help select the optimal bait molecule, immobilization strategy, and buffer conditions to maximize specificity and sensitivity.
2. Advanced Technologies
3. Comprehensive Workflow
Our in vitro pull-down assay service follows a streamlined workflow:
4. Data Analysis and Interpretation
MtoZ Biolabs provides detailed reports that include experimental results, data analysis, and interpretation. Our bioinformatics team can assist in identifying novel interactors and mapping interaction networks.
5. Flexible Sample Requirements
We accept a wide range of sample types, including purified proteins, cell lysates, and synthetic nucleic acids. Detailed guidelines are provided to ensure optimal sample preparation and submission.
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Figure2. General schematic of a pull-down assay
Applications of In Vitro Pull-Down Assays
1. Protein Interaction Studies
2. Drug Discovery and Development
3. Functional Genomics
4. Structural Biology
5. Post-Translational Modification Studies
Assessing the impact of phosphorylation, ubiquitination, or acetylation on molecular interactions
Sample Submission Suggestions
To ensure the success of your in pull-down assay, please follow these sample preparation guidelines:
1. Purified Proteins
Provide sufficient quantities of purified bait and prey proteins, along with buffer composition details.
2. Cell Lysates
Submit lysates prepared using compatible lysis buffers, avoiding harsh detergents or inhibitors.
3. Nucleic Acids
Provide high-quality DNA or RNA samples, including details of any modifications.
4. Small Molecules
Submit compounds in appropriate solvents, with details of their solubility and stability.
If you have any special requirements or need assistance with sample preparation, please contact us.
Partner with MtoZ Biolabs for your in vitro pull-down assay needs. Contact us to discuss your project and receive a customized solution that accelerates your research. With our expertise and state-of-the-art facilities, we are your trusted partner in advancing scientific discovery.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
Pull Down based Protein Analysis Service with Mass Spectrometry
Fusion Protein Interaction Analysis Service | Pull-Down and MS
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