Deep DIA Proteomics Service

Deep data-independent acquisition (Deep DIA) proteomics is an advanced technique utilizing the Astral mass spectrometry platform to facilitate efficient differential quantitative proteomics analysis via a data-independent acquisition mode. This method employs Narrow Window direct data acquisition, enabling rapid quantitative analysis and comprehensive proteomics investigations at high sequencing depths. By combining quadrupole Orbitrap mass spectrometry with the Astral platform, Deep DIA offers superior scanning speeds, exceptional resolution, sensitivity, and low ppm mass accuracy.

 

Deep DIA's versatility allows for the analysis of up to 100 complete yeast proteomes per day, processing approximately 10,000 human proteins in just 30 minutes. Its analytical depth is comparable to that of next-generation RNA sequencing, achieving a tenfold increase in throughput relative to traditional mass spectrometry methods. Furthermore, the quantitative accuracy of Narrow Window DIA is significantly enhanced, allowing for the quantification of over 14,000 proteins in a single run within half an hour, thereby providing essential support for biological research.

 

 

Guzman, U. H. et al. Nat Biotechnol. 2024.

Figure 1. Benchmarking of nDIA against DDA

 

At MtoZ Biolabs, we specialize in Deep DIA Proteomics Service, utilizing cutting-edge instruments and extensive expertise to deliver high-quality proteomics data and robust support for scientific inquiry.

 

Analysis Workflow

1. Sample Preparation

Extract and purify target proteins to ensure sample integrity.

 

2. Enzymatic Digestion

Digest proteins to produce analyzable peptides.

 

3. Mass Spectrometry Analysis

Perform LC-MS/MS analysis using Deep DIA technology to capture comprehensive mass spectrometry data.

 

4. Data Processing

Employ machine learning algorithms to analyze the acquired data, enabling accurate protein identification and quantification.

 

Service Advantages

• High Detection Depth: Detects over 10,000 proteins in conventional plant and animal tissues, meeting the demands for complex sample analysis.
• Rapid Turnaround Time: Capable of processing hundreds of samples daily, significantly enhancing experimental efficiency.
• Data Stability: Ensures result consistency and repeatability, providing reliable data for future studies.
• Cost Efficiency: Optimizes analysis workflows through advanced algorithms, reducing resource usage and enhancing data analysis efficiency.

 

Applications

• Biomarker Discovery: Identifying disease-associated proteins to support early diagnosis and clinical applications.
• Drug Mechanism Research: Analyzing the impact of pharmaceuticals on the proteome, aiding in drug development and assessment.
• Disease Mechanism Exploration: Investigating protein expression changes across different disease states, revealing potential biological mechanisms.
• Basic Research: Providing substantial data support for cell and molecular biology research, advancing scientific knowledge.

 

Free project evaluation, welcome to learn more details! Our technical specialists are available to provide a free business assessment.