Selected Reaction Monitoring Mass Spectrometry
Selected reaction monitoring mass spectrometry is an analytical technique based on tandem mass spectrometry, offering high sensitivity and precise quantification. It is widely utilized in proteomics, metabolomics, pharmacokinetics, and environmental science. By leveraging the high selectivity of tandem mass spectrometry, SRM enables highly specific detection through precise selection of precursor ions and their characteristic fragment ions. Compared to conventional full-scan approaches, SRM significantly reduces background noise, enhances sensitivity, and ensures accurate quantification of low-abundance biomolecules, even in complex matrices. As a result, it has become an essential tool for protein quantification, drug residue analysis, and biomarker research.
Selected reaction monitoring mass spectrometry primarily relies on triple quadrupole mass spectrometers. The technique's core strength lies in the ability of the triple quadrupole system to isolate and analyze specific ion pairs: the first quadrupole (Q1) selects the precursor ion, the second quadrupole (Q2) fragments it via collision-induced dissociation (CID), and the third quadrupole (Q3) detects specific fragment ions. This dual mass filtering process ensures exceptional quantitative precision, making SRM particularly effective for targeted protein and metabolite analysis.
Despite its advantages in specificity and accuracy, selected reaction monitoring mass spectrometry presents certain challenges. First, optimizing precursor-product ion pairs and collision energy for each target molecule requires extensive method development, limiting its applicability to targeted analysis rather than unknown molecule screening. Additionally, because SRM relies on predefined ion pairs, its throughput is constrained, making simultaneous detection of a large number of molecules difficult.
MtoZ Biolabs specializes in providing high-quality MRM/PRM quantitative proteomics services, including protein quantification, metabolite analysis, and drug metabolism studies. Equipped with cutting-edge mass spectrometry platforms and optimized analytical methodologies, we deliver high-sensitivity and high-specificity quantification solutions, ensuring data accuracy and reliability.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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