Deep Blood 4D-DIA Proteomics Service

Deep blood 4D-DIA proteomics is an innovative omics technology designed for the in-depth analysis of low-abundance proteins within blood samples. Blood is the most frequently tested specimen in clinical practice, and its protein alterations serve as critical biomarkers for various diseases, offering essential information for clinical diagnostics. Recent advancements in mass spectrometry have facilitated the screening of novel blood biomarkers through omics approaches, positioning mass spectrometry-based high-throughput blood proteomics as a vital tool in tumor research, immune disorders, cardiometabolic diseases, and reproductive health.

 

MtoZ Biolabs offers Deep Blood 4D-DIA Proteomics Service, which combines the benefits of nanoparticle-specific enrichment for low-abundance proteins with the data-independent acquisition (DIA) methodology to enable comprehensive blood sample analysis. This specialized enrichment technique greatly enhances the identification depth of low-abundance proteins, thereby advancing biomarker discovery and enabling tumor liquid biopsy applications. Through the refinement of sample preparation methods, mass spectrometry protocols, and data analysis techniques, our process can identify over 2000 proteins in a single blood sample, with the potential to reach 3000, while multi-sample analyses can identify more than 4000 proteins, making it ideal for clinical investigations.

 

Analysis Workflow



 

Service Advantages

1. Medium and Low-Abundance Protein Coverage

Our nanoparticle technology facilitates the detection of medium and low-abundance proteins, significantly improving the comprehensiveness of the analysis.

 

2. High Depth Identification

Our 4D-DIA technology allows for the identification of over 1500-2000 proteins, providing a robust data foundation for further research.

 

3. First-Class Mass Spectrometry Platform and Software

Utilizing the timsTOF pro 2 mass spectrometry platform along with the upgraded Spectronaut software ensures high sensitivity and accuracy while enhancing data analysis efficiency and reliability.

 

Sample Submission Requirements

Blood samples should be no less than 1 mL, and serum/plasma samples should be at least 200 μL. For more detailed requirements regarding sample submission, please contact our technical support.

 

Applications

1. Disease Biomarker Research: Identifying proteins linked to specific diseases to facilitate early diagnosis and personalized treatment strategies.

2. Drug Development: Monitoring the impact of pharmaceuticals on the proteome during drug development, offering data for safety and efficacy assessments.

3. Biomarker Validation: Providing quantitative analysis and validation for potential biomarkers, thus enhancing the reliability and validity of research findings.

4. Basic Medical Research: Investigating the roles and interactions of blood proteins, contributing new insights and methodologies to basic medical research.

 

Deep Blood 4D-DIA Proteomics represents a cutting-edge and powerful tool in life science research, enabling detailed analysis of complex protein profiles in blood samples. MtoZ Biolabs is dedicated to delivering expert services to our clients, advancing research in related fields. For further information or collaboration opportunities, please do not hesitate to contact us.