How to Use GFP Tags for Co-Immunoprecipitation

Express GFP fusion, lyse gently at 4°C with inhibitors, incubate GFP-Trap beads, wash stringently, elute for Western blot or LC-MS/MS interaction mapping.

Key takeaways

• Tag position affects function—test N- or C-terminal fusions.
• GFP-Trap gives high-affinity capture.
• Reduce background with extra washes and mild detergent.
• Co-IP/MS scales to hundreds of interactors.







Related services

Co-IP and interactions Co-Immunoprecipitation Protein Interaction Analysis Service Co-IP/Mass Spectrometry Service Immunoprecipitation Mass Spectrometry Service Protein Interaction Analysis Service

Quantitative follow-up Label-Free Quantitative Proteomics Service Affinity Purification Mass Spectrometry Service Pull-Down Assay Service Protein Interaction Network Analysis Service

FAQ

GFP vs epitope tags? GFP allows fluorescence tracking plus universal capture reagents.

Low capture efficiency? Check fusion integrity and tag placement.

Conclusion

GFP Co-IP is a versatile entry point to validate and discover protein interaction partners.