Pre-Isolated Exosome Protein Identification
CHOOSE THIS ROUTE WHEN You have a pre-isolated exosome preparation, protein lysate, or prepared peptides and need discovery-scale protein identification.
Click to preview →
Exosome Proteomics by LC-MS/MS
Exosome Protein Analysis Service
Characterize proteins in exosome preparations, compare protein abundance across defined study groups, and prioritize discovery-stage candidates using LC-MS/MS proteomics.
MtoZ Biolabs supports exosome proteomics projects from sample and preparation assessment through protein identification, quantitative analysis, data interpretation, and reporting.
- Start with plasma, serum, urine, CSF, conditioned medium, pre-isolated exosome preparations, protein lysates, or prepared peptides.
- Choose protein identification or Label-Free, TMT/iTRAQ, or DIA-based quantitative proteomics based on the study objective.
- Evaluate selected candidate proteins for PRM-based targeted MS, western blotting, or ELISA follow-up when technically suitable.
Sample PreparationProtein IdentificationQuantitative Proteomics
Research Experience
Exosome Proteomics Support for Academic and Industry Research
Projects can be configured for different sample sources, preparation stages, and proteomic study objectives.
When This Service Is a Good Fit
This service is suited to exosome-focused studies that require protein identification, quantitative comparison, or an integrated route from source material or pre-isolated preparations to LC-MS/MS analysis.
Exosome Protein Identification
Identify proteins in submitted exosome preparations to characterize their protein composition.
Comparative Exosome Proteomics
Compare protein abundance in exosome preparations across treatment, disease, time-point, or experimental model groups.
From Source Material to Proteomics
Start from plasma, serum, urine, CSF, or conditioned medium when vesicle isolation or enrichment is needed before an exosome-focused proteomics analysis.
Exosome Characterization Before Proteomics
Combine NTA, TEM/Cryo-EM, or marker protein analysis with downstream proteomics when preparation quality or characterization needs to be assessed.
Cell Culture-Derived Exosome Studies
Analyze protein changes in conditioned-medium-derived preparations across defined experimental conditions.
Pre-Isolated Samples Requiring Review
Review submitted vesicle preparations when the isolation method, particle data, marker results, or preparation history are incomplete.
Choose the Right Exosome Proteomics Workflow
Select a workflow based on the material available for submission and whether the study requires protein identification or quantitative comparison.
Not sure which route fits? Provide your sample type, preparation status, study groups, and research objective in the project form. We can recommend an appropriate workflow.
Source Material to Exosome Protein Identification
Choose this route when Your project starts with plasma, serum, urine, CSF, or conditioned medium and requires vesicle isolation or enrichment before LC-MS/MS protein identification.
Click to preview →Pre-Isolated Exosome Quantitative Proteomics
Choose this route when You have pre-isolated exosome preparations from defined study groups and need quantitative comparison of their protein profiles.
Click to preview →Source Material to Exosome Quantitative Proteomics
Choose this route when Your project starts with grouped source materials and requires a consistent vesicle preparation workflow before quantitative proteomic comparison.
Click to preview →
Workflow preview
Exosome Protein Analysis Workflow
Choose a workflow based on your starting material and whether the project requires protein identification or quantitative comparison. Sample preparation and characterization can be incorporated when needed.
Exosome Proteomics Workflow
A project workflow from sample assessment and preparation through LC-MS/MS analysis, data interpretation, and reporting.
Project Review
Review sample type, preparation status, study design, available material, and analysis objectives.
Sample Preparation & Characterization
Review submitted preparations or perform vesicle isolation or enrichment, purification, and selected characterization when required.
Protein Extraction & Digestion
Extract proteins and prepare peptides for bottom-up proteomic analysis.
LC-MS/MS Acquisition
Acquire protein identification or quantitative proteomics data using the selected analytical route.
Data Analysis
Perform quality assessment, protein identification and quantification, group comparison, and selected bioinformatic analyses.
Results & Reporting
Deliver project-specific results, data files, figures, analysis outputs, and reporting.
The workflow is configured according to the submitted material, preparation status, study design, and selected proteomics route.
What to Send Us
Recommended sample input depends on the submitted material and the analyses required. Requirements may be adjusted according to sample condition, preparation status, and study design.
| Sample Type | Quantitative Proteomics | EXOSOME CHARACTERIZATION |
|---|---|---|
| Cell Culture-Conditioned Medium | 50 mL | 50–100 mL |
| Plasma or Serum | 1 mL | 3 mL |
| Urine | 50 mL | 50–100 mL |
| Cerebrospinal Fluid (CSF) | 2 mL | 5 mL |
| Pre-Isolated Exosome Preparations | Evaluated based on available amount, preparation quality, buffer composition, and existing characterization information | Evaluated based on available volume, preparation method, buffer composition, and requested characterization analyses |
| Exosome Protein Lysate or Prepared Peptides | Evaluated based on protein or peptide amount, concentration, buffer composition, and LC-MS/MS compatibility | Not suitable for particle or morphology assessment. Available characterization depends on the submitted material. |
Need a Sample Feasibility Review?
If sample input is limited or preparation information is incomplete, provide the sample details before shipment for feasibility assessment. Store samples at −80°C, ship on dry ice, and avoid repeated freeze–thaw cycles.
Results & Deliverables
Deliverables are defined according to the selected exosome proteomics workflow, study design, and analysis scope.
Exosome Preparation Characterization
NTA particle-size distribution and concentration data, marker protein western blot results, and TEM or cryo-EM images when included in the project scope.
Protein Identification
Protein identification tables with accession information, protein annotation, and identification metrics.
Protein Quantification
Protein abundance matrices generated using the selected quantitative proteomics strategy.
Differential Analysis
For quantitative projects, group-comparison results, statistical analysis, and relevant visualizations based on the study design.
Functional Analysis & Candidate Prioritization
GO and KEGG annotation or enrichment analysis, PPI analysis, and prioritization of candidate proteins as applicable.
Data Files & Report
Project-specific data tables, analysis figures, method information, and a final project report.
Platform & Capabilities
Integrated sample preparation, characterization, LC-MS/MS proteomics, and data analysis for exosome-focused studies.
Project Support
Start Project Review
From Sample Review to Result Interpretation
Each project is reviewed according to the sample source, preparation status, available material, study design, and intended proteomics analysis.
Sample Review
Plasma, serum, urine, CSF, conditioned medium, pre-isolated exosome preparations, protein lysates, or prepared peptides.
Vesicle Isolation & Enrichment
Ultracentrifugation, density-gradient separation, SEC, precipitation, filtration, affinity capture, or microfluidic approaches selected according to sample type and project needs.
Characterization
NTA particle-size and concentration analysis, marker protein western blotting for CD9, CD63, and CD81, and TEM or cryo-EM morphology assessment.
Quantitative Proteomics
Label-Free, TMT/iTRAQ, or DIA-based analysis selected according to sample number, study design, and comparison goals.
Data Analysis
Protein identification and quantification, differential analysis, GO/KEGG analysis, PPI analysis, and candidate protein prioritization as applicable.
Representative Platforms
LC-MS/MS Platforms for Exosome Proteomics
Orbitrap Exploris 480
High-resolution LC-MS/MS acquisition
timsTOF Pro
Ion-mobility-enabled proteomics acquisition
Orbitrap Astral
High-performance LC-MS/MS acquisition for selected workflows
Vesicle Isolation & EnrichmentNTAMarker Protein WBLabel-FreeTMT / iTRAQDIAGO / KEGG / PPIPRM / WB / ELISA Follow-Up
Applications of Exosome Proteomics
Exosome proteomics can support protein profiling, quantitative comparison, and discovery-stage candidate prioritization across a range of research settings.
Disease and Control Comparisons
Compare protein profiles in exosome preparations from disease and control cohorts or other defined biological groups.
Treatment Response Studies
Evaluate changes in exosome protein abundance following drug treatment, stimulation, or other experimental interventions.
Time-Course Studies
Track changes in exosome protein profiles across time points, biological stages, or treatment phases.
Cell Culture Exosome Studies
Analyze proteins in exosome-focused preparations derived from conditioned media across cell lines, models, or experimental conditions.
Biofluid Exosome Studies
Profile proteins in preparations obtained from plasma, serum, urine, or CSF for exosome-focused research and group comparison.
Candidate Protein Prioritization
Prioritize discovery-stage candidate proteins based on proteomic differences, functional analysis, and the biological context of the study.
FAQs
Frequently asked questions about exosome proteomics projects, sample requirements, preparation, characterization, and LC-MS/MS analysis.
MtoZ Biolabs supports exosome-focused proteomics projects using plasma, serum, urine, cerebrospinal fluid (CSF), conditioned medium, and pre-isolated preparations. Protein lysates or prepared peptides can also be evaluated for downstream LC-MS/MS analysis.
Yes. Please provide the isolation or enrichment method, buffer composition, storage history, available amount, and any existing particle, marker protein, or morphology data. This information helps determine whether the submitted material is suitable for the planned proteomics workflow.
The listed amounts are recommended inputs rather than universal minimums. Projects with limited material can be reviewed according to sample type, available amount, preparation status, and planned analysis.
Samples should generally be stored at −80°C and shipped on dry ice. Repeated freeze–thaw cycles, contamination, hemolysis in applicable biofluid samples, incompatible buffers, or prolonged processing delays may affect downstream analysis.
Yes. Depending on the starting material and project requirements, available approaches include ultracentrifugation, density-gradient separation, size-exclusion chromatography, precipitation, filtration, affinity capture, and microfluidic methods. The preparation route is selected according to sample type, available input, and study objective.
Available options include NTA for particle-size distribution and concentration, TEM or cryo-EM for morphology assessment, and western blotting for marker proteins such as CD9, CD63, and CD81. The appropriate combination depends on the submitted material and project requirements.
Label-Free, TMT/iTRAQ, and DIA-based quantitative proteomics are available. Method selection depends on sample number, study design, available material, and the comparisons required.
Protein coverage varies with sample source, available input, preparation quality, background complexity, protein abundance, and the LC-MS/MS workflow used. A fixed identification number is not used as a universal service expectation.
Yes. Selected candidate proteins may be considered for targeted mass spectrometry, western blotting, or ELISA follow-up when technically suitable. The appropriate validation route depends on the candidate protein, sample matrix, and study objective.