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Ubiquitin-proteomics

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Overview

Ubiquitination is an important PTM in cell biology, as the ubiquitin-proteasome complex has mediated degradation of 80-85 percent proteins, and directly affects the expression level and function of proteins. MtoZ Biolabs is glad to offer ubiquitination identification and quantification service through high-resolution mass spectrometry analysis. For some low abundance ubiquitin-proteins, we can provide peptide enrichment service before mass spectrometry analysis. To ensure high specificity and efficacy of enrichment of ubiquitin-peptides, we use CST Ubiquitin antibody for enrichment process, and use 2-3 different enzymes for protein digestion to ensure full scan of ubiquitin-peptides.

MtoZ Biolabs has developed a specialized platform equipped with Q Exactive HF (Thermo Fisher), Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometers, equipped with Nano-LC for ubiquitin-proteomics analysis service. To better solve your research problems, we offer an all-inclusive service, including protein purification, digestion, peptide enrichment and separation, LC-MS/MS analysis, MS data analysis and bioinformatics analysis. All you need to do is to tell us your project objective, and send us your cell/protein samples, and we will complete all the following experiments.

Service Workflow

Key Features

• High-throughput analysis, identify and quantify up thousands of proteins at once
• Quantitatively analyze low abundance ubiquitin-proteins
• Use 2-3 enzymes for protein digestion, ensure full-coverage analysis
• State-of-art Nano LC and Orbitrap Fusion Mass Spectrometer

Sample Requirement

*Note: We will perform testing experiments before we start official experiments. To ensure the most cost-effective and accurate analysis is provided, only qualified samples will proceed to the official analysis.

Reports

• Experiment procedures
• Parameters of liquid chromatography and mass spectrometer
• MS raw data files
• Peptide identifications and intensities
• Protein identifications and ubiquitin mapping
Bioinformatics analysis

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Address:
155 Federal Street, Suite 700, Boston, MA 02110, USA
Email:
info@MtoZ-Biolabs.com
Tel:
1-857-928-2754