Resources

Proteomics Databases



Metabolomics Databases

• • Main Types of Protein Glycosylation: N-Linked, O-Linked, and GPI Anchors Explained

  A practical guide to the main types of protein glycosylation—N-linked, O-linked, and GPI anchors—including biosynthesis, consensus motifs, biological roles, and mass spectrometry analysis approaches.

• • Cell Sequencing: Single-Cell and Population-Level Workflows, Applications, and Data Analysis

  A practical guide to cell sequencing, covering single-cell sequencing, population cell sequencing, sample preparation, isolation methods, WGA, WTA, NGS library construction, data analysis, and biomedical applications.

• • How to Determine Amino Acids from a Mass Spectrum and Locate Modification Sites

  A practical guide to interpreting MS/MS spectra for amino acid assignment, residue mass differences, post-translational modification detection, and modification site localization in proteomics.

• • Activity-Based Protein Profiling in Drug Discovery: Workflow, Applications, and Method Selection

  A practical guide to activity-based protein profiling (ABPP) for drug discovery, covering activity probes, LC-MS/MS, target engagement, competitive profiling, and chemical proteomics workflows.

• • Bottom-Up Proteomics in Single-Cell Proteomics: Challenges, Workflow, and Method Selection

  A practical guide to bottom-up single-cell proteomics, covering sample preparation, TMT multiplexing, carrier boost, LC-MS/MS, DIA vs DDA, missing values, and emerging opportunities.

• • Histone Butyrylation (Kbu): Butyrate Metabolism, Epigenetic Regulation, and LC-MS/MS Detection

  A practical guide to histone butyrylation (Kbu), including butyryl-CoA formation, links to butyrate metabolism, lysine acylation biology, writers and erasers, disease relevance, and LC-MS/MS detection.

• • LC-MS Histone Modification Detection: PTM Signatures, Workflows, and Quantification

  A practical guide to LC-MS histone modification detection, covering histone extraction, peptide preparation, enrichment, acetylation, methylation, phosphorylation, mass shifts, site localization, and quantitative PTM analysis.

• • DIA vs DDA Proteomics: Acquisition Strategy, Quantification, and Method Selection

  A practical comparison of DIA and DDA proteomics, including precursor selection, MS/MS complexity, reproducibility, missing values, spectral libraries, quantitative accuracy, and application scenarios.

• • Main Types of Protein Glycosylation: N-Linked, O-Linked, GPI Anchors, and Glycoproteomics

  A practical guide to the main types of protein glycosylation, including N-linked glycosylation, O-linked glycosylation, GPI anchors, C-mannosylation, glycation, site analysis, and LC-MS/MS glycoproteomics.

• • How to Identify Amino Acids and Modification Sites from a Mass Spectrum

  A practical guide to reading peptide mass spectra, using residue mass differences, b/y ions, neutral losses, database search, de novo sequencing, and PTM localization to identify amino acids and modification sites.