Resources

Proteomics Databases



Metabolomics Databases

• • How to Quantify Histone Malonylation Levels?

  With the continuous advancement of metabolic epigenetics, research on histone post-translational modifications has progressively shifted from determining whether modifications occur to understanding how modification levels dynamically fluctuate under different biological conditions. Among these modifications, histone malonylation has emerged as a critical regulatory mechanism linking cellular metabolism to chromatin regulation, making it a major focus in the interdisciplinary fields of proteomics and ......

• • How to Perform Histone Malonylation Proteomics Analysis?

  Histone malonylation is an emerging histone acylation modification that has gained considerable attention in epigenetics and metabolic research in recent years. By introducing malonyl groups onto lysine residues of histones, histone malonylation can alter chromatin architecture and regulate gene expression, thereby providing new insights into the interplay between metabolism and epigenetic regulation. To comprehensively elucidate the biological functions of histone malonylation, proteomics analysis ha......

• • How to Analyze PhIP-Seq Data: From Peptide Enrichment to Antibody Signature Interpretation

  A practical workflow for PhIP-Seq analysis, covering count normalization, peptide enrichment, hit calling, antigen annotation, antibody signature interpretation, and validation planning.

• • What Is PhIP-Seq? Principles, Applications, Strengths, and Study Design Considerations

  Learn how PhIP-Seq profiles antibody binding with phage-displayed peptide libraries, NGS readout, enrichment analysis, and study design planning for antigen discovery.

• • VirScan and PhIP-Seq: How Viral Peptide Libraries Support Immune History and Serology Studies

  Compare VirScan-style viral peptide PhIP-Seq, custom pathogen-focused PhIP-Seq, and conventional serology for immune history, vaccine-response profiling, and viral antigen discovery.

• • PhIP-Seq Workflow Explained: Library Design, Sample Incubation, Sequencing, QC, and Data Review

  A practical PhIP-Seq workflow diagram for planning peptide library design, sample incubation, immunoprecipitation, sequencing, QC, enrichment analysis, and peptide-level data review.

• • What Are the Technical Challenges in Histone Propionylation Analysis?

  Histone propionylation, an important type of short-chain lysine acylation on histones, has increasingly attracted research interest in studies of epigenetic regulation and its coupling with cellular metabolism. This modification has been implicated in the regulation of chromatin structure, control of gene transcription activity, and cellular responses to metabolic states. However, because histone propionylation is typically of low abundance and structurally very similar to acetylation, butyrylation, a......

• • What Is H3K27ac and Why Is It a Popular Epigenetic Mark?

  In complex gene regulatory networks, the DNA sequence alone cannot fully account for the dynamic changes in gene expression, and epigenetic modifications play a crucial role. Histone modifications, as a major form of epigenetic regulation, directly influence gene expression by altering chromatin structure and modulating transcription factor accessibility. Notably, H3K27ac (acetylation of lysine 27 on histone H3) has become a key marker in functional genomics research due to its strong association with......

• • How to Isolate and Enrich Mitochondria for Proteomics Analysis?

  In modern life science research, mitochondria are recognized not only as cellular powerhouses but also as central regulators of cellular metabolism, apoptosis signaling, and disease development. Advances in mass spectrometry have enabled detailed analyses of the mitochondrial proteome, metabolome, and lipidome. However, the low abundance of mitochondria within cells and the presence of complex cellular backgrounds make mitochondrial enrichment a critical step for high-quality mass spectrometry analysi......

• • What Is Histone Acetylation Analysis?

  Histone acetylation refers to the post-translational addition of acetyl groups to lysine residues on histone proteins catalyzed by histone acetyltransferases. This modification alters chromatin structure and regulates gene transcriptional activity. By neutralizing the positive charge of lysine residues, histone acetylation weakens the electrostatic interaction between DNA and histones, leading to a transition of chromatin from a condensed to an open configuration. As a fundamental component of epigene......