Resources

Proteomics Databases



Metabolomics Databases

• • Applications and Value of PRM Mass Spectrometry in Protein Quantification

  Although immunoassay-based methods such as ELISA and Western blot are widely employed, they exhibit inherent limitations in specificity, throughput, and quantitative accuracy. These constraints become particularly evident when analyzing complex biological matrices and low-abundance proteins. Consequently, there is a growing demand for analytical approaches that offer high sensitivity, robust reproducibility, and accurate quantification to support applications such as biomarker validation, drug target ......

• • Advantages and Disadvantages of High-Throughput Ubiquitinomics

  Ubiquitination, as one of the most important post-translational modifications, is widely involved in biological processes such as cell cycle regulation, DNA repair, signal transduction, and protein degradation, and serves as a central mechanism for maintaining cellular homeostasis and regulating signaling pathways. However, ubiquitination is characterized by low abundance, rapid turnover, and complex chain architectures, which have long hindered its systematic and high-resolution characterization. Tra......

• • Beginner’s Guide to Acetylproteomics Research: Sample Prep and Analysis Tips

  As a major type of protein post-translational modification (PTM), acetylation is extensively involved in fundamental biological processes, including cellular metabolism, signal transduction, and gene expression regulation. In particular, in studies of histone regulation, mitochondrial function, and metabolic reprogramming, protein acetylation has emerged as a cutting-edge area for elucidating disease mechanisms and identifying potential therapeutic targets. With advances in high-resolution mass spectr......

• • Mechanisms and Pathways of Protein Ubiquitination

  Within complex cellular systems, the dynamic regulation of protein function represents a fundamental mechanism for maintaining cellular homeostasis. Protein ubiquitination, a highly conserved and reversible post-translational modification, is broadly involved in diverse biological processes, including cell cycle regulation, stress responses, DNA repair, and signal transduction. By covalently tagging substrate proteins, ubiquitination governs their stability, activity, subcellular localization, and int......

• • How to Enrich Golgi Proteins for Proteomic Analysis?

  The Golgi apparatus, a central membranous organelle within cells, plays essential roles in protein modification, sorting, and transport. It is critical for cellular signaling, the secretory system, and understanding disease mechanisms. Therefore, precise identification and quantitative analysis of Golgi proteins are crucial for elucidating cellular functions and disease processes. However, due to the low abundance of Golgi proteins within total cellular proteins, their enrichment and isolation remain ......

• • How to Enrich Lactylated Peptides for Mass Spectrometry?

  Lactylation is an emerging post-translational modification, first identified in 2019, which modulates protein structure and function by introducing a lactyl group (-CO-CH₃-OH) onto lysine residues. Lactylation plays critical roles in regulating carbohydrate metabolism, inflammatory responses, and tumorigenesis. For instance, histone lactylation can modulate chromatin accessibility, thereby influencing gene expression, while non-histone lactylation participates in the regulation of cellular metabolic p......

• • Can Histone Kbu Serve as a Novel Epigenetic Biomarker?

  In life science research, epigenetics has emerged as a central field for elucidating mechanisms underlying gene expression regulation. In recent years, it has been demonstrated that diverse post-translational modifications of histones not only modulate chromatin architecture but also play essential roles in gene activity, cell fate determination, and disease progression. Among these modifications, histone lysine butyrylation (Kbu, Lysine Butyrylation), as a newly identified acyl modification, has attr......

• • How to Detect Histone Malonylation Modifications?

  Histones, as fundamental components of chromatin, play a central role in the regulation of gene expression and cell fate determination through a variety of post-translational modifications. In addition to classical modifications such as acetylation, methylation, and phosphorylation, histone malonylation has emerged in recent years as an important focus in epigenetic research. This modification involves the covalent attachment of a malonyl group to lysine residues, thereby influencing chromatin accessi......

• • Untargeted Metabolomics Analysis: Principles, Applications, and Workflow

  In the context of the continuous evolution of systems biology, metabolomics has emerged as a key discipline for interpreting dynamic changes in biological processes. Among its subfields, untargeted metabolomics plays a central role in both basic research and translational medicine due to its capability to perform global, unbiased profiling of metabolites without predefined targets. Compared with targeted approaches, untargeted strategies emphasize discovery and enable the capture of potentially critic......

• • How to Map the Golgi Proteome Using Mass Spectrometry?

  In cell biology, the Golgi apparatus is a central organelle involved in protein processing, modification, and sorting, and its dysfunction is closely associated with a range of diseases, including neurodegenerative disorders and cancer. In recent years, rapid advances in mass spectrometry and proteomics have enabled systematic characterization of Golgi protein composition and its dynamic alterations at a systems level. Significance of Golgi Proteome Research The Golgi apparatus not only participates ......