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Proteomics Databases



Metabolomics Databases

• • How to Detect Histone Ubiquitination?

  Histone ubiquitination is a crucial post-translational modification involved in chromatin regulation. By covalently attaching ubiquitin molecules to specific residues on histones, it modulates gene expression, DNA damage repair, and chromatin dynamics. Due to the low abundance of ubiquitinated histones and their susceptibility to deubiquitinases, accurate detection presents a significant challenge. Researchers commonly employ a combination of Western blotting, immunoprecipitation, and mass spectrometr......

• • How to Analyze Histone Ubiquitination in Cancer Samples?

  Histone ubiquitination is a critical epigenetic modification that regulates chromatin architecture and consequently influences gene expression, DNA damage repair, and tumor initiation and progression. In cancer research, aberrant histone ubiquitination has been closely associated with tumor progression, therapeutic resistance, and alterations in the tumor immune microenvironment. Therefore, accurate characterization of histone ubiquitination is of considerable importance for identifying novel therapeu......

• • What Factors Affect the Reliability of Histone Crotonylation Analysis?

  Histone crotonylation is a recently identified lysine post-translational modification (PTM) that plays critical roles in transcriptional regulation, cell fate determination, and disease pathogenesis. With continuous advances in mass spectrometry technologies, studies of histone crotonylation have expanded substantially. However, because crotonylation is a low-abundance and dynamically reversible modification, the reliability of analytical results can be significantly influenced by multiple factors, in......

• • What Are the Technical Challenges in Histone Crotonylation Analysis?

  Histone crotonylation (Kcr) is an emerging lysine post-translational modification (PTM) that plays important roles in gene transcription regulation, cellular differentiation, and stress responses. Although its biological significance is increasingly being elucidated, the inherently low abundance of Kcr, its chemical instability, and the coexistence of multiple modification sites present substantial challenges for both qualitative and quantitative analyses. Mass spectrometry (MS) remains the primary an......

• • High-Sensitivity HCP Quantification: Label-Based vs Label-Free Approaches

  Host Cell Proteins (HCPs) are endogenous protein impurities derived from expression systems, such as CHO cells, Escherichia coli, and yeast, during the manufacture of biopharmaceutical products. Even at trace levels, certain HCPs may induce immunogenic responses, compromise product stability, or adversely affect product safety. Consequently, accurate HCP quantification represents a critical component of biopharmaceutical quality control. High-sensitivity HCP detection is equally important during proc......

• • Technologies and Advantages of Targeted Metabolomics

  With the continued advancement of life science research, metabolomics has emerged as a critical approach for characterizing dynamic changes in metabolites across cells, tissues, and biofluids. It plays an essential role in elucidating disease mechanisms, biomarker discovery, and drug target identification. Among its subfields, targeted metabolomics has gained increasing attention in clinical translational research, pharmacodynamic evaluation, and precision medicine due to its high sensitivity, strong ......

• • What Is Histone Ubiquitination?

  In life science research, histone modifications have long been a central focus in studies of gene expression regulation and chromatin dynamics. In addition to well-characterized modifications such as methylation, acetylation, and phosphorylation, increasing attention has recently been directed toward a specific post-translational modification, histone ubiquitination. This modification not only contributes to the fine-tuned regulation of gene expression but also plays essential roles in DNA damage repa......

• • Application of HCP Detection in Recombinant Protein Drug Development

  In recent years, continuous advances in protein engineering and expression system optimization technologies have greatly expanded the diversity of recombinant protein therapeutics. Beyond conventional monoclonal antibodies and fusion proteins, emerging modalities such as bispecific antibodies, multispecific proteins, Fc-fusion proteins, long-acting biologics, and enzyme replacement therapies have driven rapid innovation across the biopharmaceutical industry. Despite their differences in structure and ......

• • What Samples Are Suitable for Histone Acetylation Analysis?

  Histone acetylation is a critical epigenetic modification involved in chromatin regulation, participating widely in gene transcription, the cell cycle, DNA repair, and metabolic regulation. Its dynamic changes reflect cellular states, disease progression, and responses to environmental or experimental stimuli. Highly sensitive and accurate histone acetylation analysis not only reveals epigenetic patterns within complex biological systems but also provides essential data for drug development and biomar......

• • How to Choose the Right Method for Histone Succinylation Analysis?

  As research on post-translational modifications (PTMs) continues to advance, histone succinylation has emerged as an important acylation modification that has attracted considerable attention because of its critical roles in epigenetic regulation, metabolic reprogramming, and disease pathogenesis. Studies have shown that histone succinylation can alter chromatin structure and transcriptional activity and is involved in diverse biological processes, including tumorigenesis, neurodegeneration, and infla......