Antibody Epitope Mapping

Overview

Monoclonal antibodies could recognize almost unlimited targets because their high specificity and affinity character. This ability has catapulted them as an attractive and fast-growing class of therapeutic & diagnostic agents over the past decades. Mapping the interactions between antibody (paratope) and antigen (epitope) is crucial for characterizing antibody’s mechanism of action, predicting suitable antigens, as well as securing intellectual property. A number of different approaches have been used for antibody epitope mapping, including X-ray crystallography, NMR, mutagenesis, peptide-based ELISA, hydrogen–deuterium exchange, etc. However, these methods generally require high levels of expertise and cost, or only give information on linear structures, which greatly impedes interpreting the precise nature of antibody-antigen interactions.

Our Platform

In order to meet this challenge, MtoZ Biolabs has introduced an innovative EpiLink™ technical platform. It is based on chemical cross-linking of proteins coupled with mass spectrometry analysis (CXMS) which can outline the overall protein architecture via identifying direct binding partners and localizing the binding interface. Yet, conventional CXMS may have problems detecting and analyzing interlinked peptides, the most informative category that faithfully reflects antigen-antibody interaction. Distinct from all current methods, our EpiLink™ Platform can offer multiple outstanding features:

Mass spectrometry analysis and EpiLink™ search

1. Designed specifically for identifying and interpreting of cross-linking spectra, providing higher sensitivity and accuracy
2. Able to interpret conformational epitopes in original folding at amino acid level
3. Required very small sample volume
4. Fast and efficient, with a very short lead time frame
5. Professional team with rich experience in utilizing EpiLink™ antibody epitope analysis

With the help of EpiLink™, we have successfully pinpointed the epitope information of multiple antibodies. Our state-of-the-art platform can be a robust tool to offer precise structural insights and support your research endeavors.

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